Recombinant Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
Overview
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Product name
Anti-Band 3/AE 1 antibody [EPR1426]
See all Band 3/AE 1 primary antibodies -
Description
Rabbit monoclonal [EPR1426] to Band 3/AE 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Band 3/AE 1 aa 1-100. The exact sequence is proprietary.
(Peptide available asab177142) -
Positive control
- Red blood cell lysate; Human colon and lung tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Band 3.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1426 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. -
Tissue specificity
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871). -
Involvement in disease
Ovalocytosis, Southeast Asian
Spherocytosis 4
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Cryohydrocytosis -
Sequence similarities
Belongs to the anion exchanger (TC 2.A.31) family. -
Post-translational
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated. -
Cellular localization
Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney. - Information by UniProt
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Database links
- Entrez Gene: 6521 Human
- Omim: 109270 Human
- SwissProt: P02730 Human
- Unigene: 210751 Human
- Unigene: 443948 Human
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Alternative names
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)](https://www.abcam.com/ps/products/108/ab108414/Images/ab108414-121113-anti-band-3-ae-1-antibody-epr1426-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)Image courtesy of an anonymous Abreview.ab108414 staining Band 3/AE 1 in Rhesus monkey erythroid cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol and then incubated with ab108414 at a 1/100 dilution for 1 hour. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.
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![Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)](https://www.abcam.com/ps/products/108/ab108414/Images/ab108414-121116-anti-band-3-ae-1-antibody-epr1426-western-blot.jpg)
Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)Anti-Band 3/AE 1 antibody [EPR1426] (ab108414) at 1/1000 dilution + Red blood cell lysate at 10 µg
Predicted band size: 102 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)](https://www.abcam.com/ps/products/108/ab108414/Images/ab108414-121115-anti-band-3-ae-1-antibody-epr1426-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)](https://www.abcam.com/ps/products/108/ab108414/Images/ab108414-121114-anti-band-3-ae-1-antibody-epr1426-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human lung tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
References
ab108414 has been referenced in 6 publications.
- Hao L et al. Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis. J Cell Mol Med 23:4454-4463 (2019). PubMed: 31016877
- Tougan T et al. Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. Sci Rep 8:5052 (2018). PubMed: 29567995
- Hwang S et al. Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator. Nat Commun 9:4045 (2018). PubMed: 30279493
- Saha D et al. Expression of hemoglobin-a and ß subunits in human vaginal epithelial cells and their functional significance. PLoS One 12:e0171084 (2017). ICC/IF ; Human . PubMed: 28178273
- Kurano M et al. Involvement of Band3 in the efflux of sphingosine 1-phosphate from erythrocytes. PLoS One 12:e0177543 (2017). PubMed: 28494002
- Voorberg-van der Wel A et al. A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasitePlasmodium cynomolgi. Elife 6:N/A (2017). PubMed: 29215331
