For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    band-3ae-1-antibody-epr1426-ab108414.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Blood Cell Antigens RBC Antigens
Share by email
RecombinantRabMAb

Recombinant Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

  • Datasheet
  • SDS
Reviews (1)Q&A (9)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
  • Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1426] to Band 3/AE 1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated Unconjugated

You may also be interested in

Assay
Product image
Acetylcholinesterase Assay Kit (Fluorometric - Green) (ab138872)
Assay
Product image
Nuclear yellow (Hoechst S769121) (ab138903)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-Band 3/AE 1 antibody [EPR1426]
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1426] to Band 3/AE 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Red blood cell lysate; Human colon and lung tissue
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1426
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Band 3/AE 1 antibody [EPR1426] (ab225080)
    • Alexa Fluor® 488 Anti-Band 3/AE 1 antibody [EPR1426] (ab225081)
    • Anti-Band 3/AE 1 antibody [EPR1426] - BSA and Azide free (ab236016)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Band 3/AE 1 protein (Tagged) (ab235968)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
  • Tissue specificity

    Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
  • Involvement in disease

    Ovalocytosis, Southeast Asian
    Spherocytosis 4
    Renal tubular acidosis, distal, autosomal dominant
    Renal tubular acidosis, distal, with hemolytic anemia
    Renal tubular acidosis, distal, with normal red cell morphology
    Cryohydrocytosis
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
    Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
  • Target information above from: UniProt accession P02730 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6521 Human
    • Omim: 109270 Human
    • SwissProt: P02730 Human
    • Unigene: 210751 Human
    • Unigene: 443948 Human
    • Alternative names

      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • Band 3 antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Images

    • Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
      Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
      Anti-Band 3/AE 1 antibody [EPR1426] (ab108414) at 1/1000 dilution + Red blood cell lysate at 10 µg

      Predicted band size: 102 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

      ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

      ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
      Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (7)

    Publishing research using ab108414? Please let us know so that we can cite the reference in this datasheet.

    ab108414 has been referenced in 7 publications.

    • Wang Y  et al. Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia. Oxid Med Cell Longev 2020:7834252 (2020). PubMed: 32963701
    • Hao L  et al. Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis. J Cell Mol Med 23:4454-4463 (2019). PubMed: 31016877
    • Tougan T  et al. Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. Sci Rep 8:5052 (2018). PubMed: 29567995
    • Hwang S  et al. Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator. Nat Commun 9:4045 (2018). PubMed: 30279493
    • Saha D  et al. Expression of hemoglobin-a and ß subunits in human vaginal epithelial cells and their functional significance. PLoS One 12:e0171084 (2017). ICC/IF ; Human . PubMed: 28178273
    • Kurano M  et al. Involvement of Band3 in the efflux of sphingosine 1-phosphate from erythrocytes. PLoS One 12:e0177543 (2017). PubMed: 28494002
    • Voorberg-van der Wel A  et al. A comparative transcriptomic analysis of replicating and dormant liver stages of the relapsing malaria parasitePlasmodium cynomolgi. Elife 6:N/A (2017). PubMed: 29215331

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Band 3 / AE 1 antibody [EPR1426]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rhesus monkey Cell (erythroid cells)
    Permeabilization
    No
    Specification
    erythroid cells
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.