Recombinant
RabMAb

Recombinant Anti-Band 3/AE 1 antibody [EPR1426] - BSA and Azide free (ab236016)

Overview

  • Product name

    Anti-Band 3/AE 1 antibody [EPR1426] - BSA and Azide free
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1426] to Band 3/AE 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Band 3/AE 1 aa 1-100. The exact sequence is proprietary.
    (Peptide available as ab177142)

  • Positive control

    • IHC-P: Human lung tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab236016 is a PBS-only buffer format of ab108414. Please refer to ab108414 for recommended dilutions, protocols, and image data.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. 

     

    Previously labelled as Band 3. 

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1426
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
  • Tissue specificity

    Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
  • Involvement in disease

    Ovalocytosis, Southeast Asian
    Spherocytosis 4
    Renal tubular acidosis, distal, autosomal dominant
    Renal tubular acidosis, distal, with hemolytic anemia
    Renal tubular acidosis, distal, with normal red cell morphology
    Cryohydrocytosis
  • Sequence similarities

    Belongs to the anion exchanger (TC 2.A.31) family.
  • Post-translational
    modifications

    Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
    Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
  • Information by UniProt
  • Database links

  • Alternative names

    • AE 1 antibody
    • AE1 antibody
    • Anion exchange protein 1 antibody
    • Anion exchanger 1 antibody
    • B3AT_HUMAN antibody
    • Band 3 anion transport protein antibody
    • Band 3 antibody
    • BND3 antibody
    • CD233 antibody
    • DI antibody
    • Diego blood group antibody
    • EMPB3 antibody
    • EPB3 antibody
    • Erythrocyte membrane protein band 3 antibody
    • Erythroid anion exchange protein antibody
    • FR antibody
    • Froese blood group antibody
    • RTA1A antibody
    • SLC4A1 antibody
    • Solute carrier family 4 anion exchanger member 1 antibody
    • Solute carrier family 4 member 1 antibody
    • SW antibody
    • Swann blood group antibody
    • Waldner blood group antibody
    • WD antibody
    • WD1 antibody
    • WR antibody
    • Wright blood group antibody
    see all

Images

  • ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108414).

  • ab108414 staining Band 3/AE 1 in Rhesus monkey erythroid cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol and then incubated with ab108414 at a 1/100 dilution for 1 hour. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108414).

  • ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108414).

References

ab236016 has not yet been referenced specifically in any publications.

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