Recombinant
RabMAb

Recombinant Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070)

Overview

  • Product name

    Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424]
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1424] to Band 3/AE 1 (phospho Y21)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Band 3/AE 1. The exact sequence is proprietary.

  • Positive control

    • Human red blood cell lysates, Pervanadate treated.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Band 3. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1424
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function

      Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • Tissue specificity

      Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • Involvement in disease

      Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • Sequence similarities

      Belongs to the anion exchanger (TC 2.A.31) family.
    • Post-translational
      modifications

      Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • Cellular localization

      Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • Database links

    • Alternative names

      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • Band 3 antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Images

    • All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (purified)

      Lane 1 : Untreated RBC
      Lane 2 : RBC treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 101 kDa
      Observed band size: 101 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution (unpurified)

      Lane 1 : Human red blood cell lysate, untreated
      Lane 2 : Human red blood cell lysate, Pervanadate treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 101 kDa

    References

    ab125070 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for contacting us. I am sorry for the confusion. Please select according to the following options.

    If the antibody was used in Immunofluorescence on cells then select ICC/IF.

    If on frozen tissue then select IHC-Fr

    If used on paraffin embedded tissues then select IHC-P

    If used in paraformaldehyde perfused and frozen sections then select IHC-FoFR.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
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    Answer

    Thank you for contacting us.

    Your customer number is XXXXXXXX.

    Please use the same email address to retrieve the password by clicking forget password.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question
    Answer

    Thank you for your email.

    You need to write the discount code when submitting the Abreview in additional notes box. Please buy the antibody without any hesitation.

    Best of luck for your research.

    Read More

    Answer

    Thank you for accepting my offer.

    Unfortunately you would need the discount code before the purchase. Could you tell me which antibody you would like to buy?

    Read More

    Answer

    Thank you for contacting us.

    The macaca Band 3 protein is about 94% homologous to the human protein. It seems that the anti human Band 3 antibodies will cross react with Macaca proteins also.

    You can choose any of the anti Band 3 antibodies from the website. Please be advised that Macaca is not a tested species so we could not 100% confirm the x-reactivity. The use of this antibody with macaca is not guaranteed however we are happy to provide the Abreview 100% testing discount if you are interested in using any of these antibody with Macaca mulata species. Please click the link for more info.

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Let me know if you are interested in my offer. I will be happy sending you the code.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Answer

    Thank you very much for your phone call.

    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytoplasmic domain of the protein.

    As discussed please send me the sequence link of Rhesus Band 3 protein, I will then do the further search.

    I hope this information will be helpful. Should you have any other question please do not hesitate to contact me.

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