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RecombinantRabMAb

Recombinant Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)
  • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)

View more associated products

Overview

  • Product name

    Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free
    See all Band 3/AE 1 primary antibodies
  • Description

    Rabbit monoclonal [EPR1427Y] to Band 3/AE 1 (phospho Y359) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Band 3/AE 1 (phospho Y359).

  • General notes

    ab247492 is the carrier-free version of ab77236 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab247492 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1427Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Cardiovascular
    • Blood
    • Other

Associated products

  • Alternative Versions

    • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Recombinant Protein

    • Recombinant Human Band 3/AE 1 protein (Tagged) (ab235968)

Applications

Our Abpromise guarantee covers the use of ab247492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 102 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • Tissue specificity

      Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • Involvement in disease

      Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • Sequence similarities

      Belongs to the anion exchanger (TC 2.A.31) family.
    • Post-translational
      modifications

      Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • Cellular localization

      Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Target information above from: UniProt accession P02730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6521 Human
      • Omim: 109270 Human
      • SwissProt: P02730 Human
      • Unigene: 210751 Human
      • Unigene: 443948 Human
      • Alternative names

        • AE 1 antibody
        • AE1 antibody
        • Anion exchange protein 1 antibody
        • Anion exchanger 1 antibody
        • B3AT_HUMAN antibody
        • Band 3 anion transport protein antibody
        • Band 3 antibody
        • BND3 antibody
        • CD233 antibody
        • DI antibody
        • Diego blood group antibody
        • EMPB3 antibody
        • EPB3 antibody
        • Erythrocyte membrane protein band 3 antibody
        • Erythroid anion exchange protein antibody
        • FR antibody
        • Froese blood group antibody
        • RTA1A antibody
        • SLC4A1 antibody
        • Solute carrier family 4 anion exchanger member 1 antibody
        • Solute carrier family 4 member 1 antibody
        • SW antibody
        • Swann blood group antibody
        • Waldner blood group antibody
        • WD antibody
        • WD1 antibody
        • WR antibody
        • Wright blood group antibody
        see all

      Images

      • Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)
        Western blot - Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)
        All lanes : Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution

        Lane 1 : Red blood cell lysate, untreated.
        Lane 2 : Red blood cell lysate treated with pervanadate.

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 102 kDa
        Observed band size: 102 kDa



        This data was developed using ab77236, the same antibody clone in a different buffer formulation.

         

      • Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)
        Anti-Band 3/AE 1 (phospho Y359) antibody [EPR1427Y] - BSA and Azide free (ab247492)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

    • References (0)

      Publishing research using ab247492? Please let us know so that we can cite the reference in this datasheet.

      ab247492 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab247492.
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