Recombinant Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
![Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-8-ab129184ICCIF1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7668] to BANF1/BAF - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Dog, Human
Overview
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Product name
Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free
See all BANF1/BAF primary antibodies -
Description
Rabbit monoclonal [EPR7668] to BANF1/BAF - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NIH/3T3 cells. Flow Cyt: SH-SY5Y and NIH/3T3 cells. WB: PC-12, Jurkat, U-87 MG, HeLa and NIH/3T3 cell lysate. IHC-P: Human astrocytoma, colon carcinoma, and glioma tissues.
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General notes
ab240953 is the carrier-free version of ab129184 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab240953 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as BANF1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7668 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 10 kDa. |
Target
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Function
Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. -
Tissue specificity
Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes. -
Sequence similarities
Belongs to the BAF family. -
Domain
Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides. -
Post-translational
modificationsPartially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA. -
Cellular localization
Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. - Information by UniProt
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Database links
- Entrez Gene: 8815 Human
- Omim: 603811 Human
- SwissProt: O75531 Human
- Unigene: 433759 Human
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Alternative names
- BAF antibody
- BAF_HUMAN antibody
- BANF 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (7.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-7-ab129184IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (0.78 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184) -
![OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-6-BANF1Primaryantibodiesab1291843.jpg)
OI-RD Scanning - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-5-BANF1Primaryantibodiesab1291842.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)ab129184, at a 1/100 dilution, staining BANF1/BAF in paraffin embedded human colonic carcinoma tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-4-ab1291847ab129184FC1.jpg)
Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF (red) with ab129184 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
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![Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-3-ab129184236567129184ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
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![Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-2-ab129184236569129184ICCIF2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-1-ab129184240732ab129184ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)This image is courtesy of an anonymous Abreviewab129184 staining BANF1/BAF in MDCK (Canine kidney cell line) cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)
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![Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)](https://www.abcam.com/ps/products/240/ab240953/Images/ab240953-9-benefits-of-recombinant-antibodies.png)
Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240953 has not yet been referenced specifically in any publications.
