Recombinant
RabMAb

Recombinant Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

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Overview

  • Product name

    Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free
    See all BANF1/BAF primary antibodies

  • Description

    Rabbit monoclonal [EPR7668] to BANF1/BAF - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

    See IHC antigen retrieval protocols.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Dog, Human

  • Immunogen

    Synthetic peptide within Human BANF1/BAF. The exact sequence is proprietary.
    Database link: O75531

  • Positive control

    • ICC/IF: NIH/3T3 cells. Flow Cyt: SH-SY5Y and NIH/3T3 cells. WB: PC-12, Jurkat, U-87 MG, HeLa and NIH/3T3 cell lysate. IHC-P: Human astrocytoma, Human colon carcinoma, and Human glioma tissues.

  • General notes

    ab240953 is the carrier-free version of ab129184 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240953 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as BANF1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.18 x 10 -10 M
    Learn more about KD

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7668

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 10 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.

    • Tissue specificity

      Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.

    • Sequence similarities

      Belongs to the BAF family.

    • Domain

      Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
      LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.

    • Post-translational
      modifications

      Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.

    • Cellular localization

      Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
    • Information by UniProt

    • Database links

      • Alternative names

        • BAF antibody
        • BAF_HUMAN antibody
        • BANF 1 antibody
        • BANF1 antibody
        • Barrier to autointegration factor 1 antibody
        • Barrier to autointegration factor antibody
        • Barrier-to-autointegration factor antibody
        • BCRG 1 antibody
        • BCRG1 antibody
        • BCRP 1 antibody
        • BCRP1 antibody
        • Breakpoint cluster region protein 1 antibody
        • D14S1460 antibody
        • MGC111161 antibody
        • NGPS antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (7.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling BANF1/BAF with Purified ab129184 at 1:100 dilution (0.78 µg/ml). Heat mediated antigen retrieval was performed using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Other - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Other - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        ab129184, at a 1/100 dilution, staining BANF1/BAF in paraffin embedded Human colonic carcinoma tissue by Immunohistochemistry.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

        Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Flow Cytometry - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF (red) with ab129184 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cells) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)

        Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma) cells labeling BANF1/BAF with ab129184 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing mainly nuclear with weakly cytoplasmic staining on Hela cell line. The nuclear counterstain is DAPI (blue).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (ab240953)This image is courtesy of an anonymous Abreview

        ab129184 staining BANF1/BAF in MDCK cell pellets in paraffin by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cell pellets were fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 1% serum for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris/EDTA pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129184)

      References

      ab240953 has not yet been referenced specifically in any publications.

      Publishing research using ab240953? Please let us know so that we can cite the reference in this datasheet.

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