Overview

  • Product name

    Anti-BANF1/BAF antibody [EPR7669]
    See all BANF1/BAF primary antibodies
  • Description

    Rabbit monoclonal [EPR7669] to BANF1/BAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BANF1/BAF aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa, HepG2 and 293T cell lysate; Human thyroid gland carcinoma tissue.
  • General notes

     

     

     This product was previously labelled as BANF1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7669
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
    • Tissue specificity

      Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the BAF family.
    • Domain

      Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
      LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
    • Post-translational
      modifications

      Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
    • Cellular localization

      Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAF antibody
      • BAF_HUMAN antibody
      • BANF 1 antibody
      • BANF1 antibody
      • Barrier to autointegration factor 1 antibody
      • Barrier to autointegration factor antibody
      • Barrier-to-autointegration factor antibody
      • BCRG 1 antibody
      • BCRG1 antibody
      • BCRP 1 antibody
      • BCRP1 antibody
      • Breakpoint cluster region protein 1 antibody
      • D14S1460 antibody
      • MGC111161 antibody
      • NGPS antibody
      see all

    Images

    • All lanes : Anti-BANF1/BAF antibody [EPR7669] (ab129074) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 10 kDa
      Observed band size: 10 kDa

    • Overlay histogram showing HepG2 cells stained with ab129074 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129074, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab129074, at 1/100 dilution, staining BANF1/BAF in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.

    References

    ab129074 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Loading amount
    50 µg
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C

    Dr. Paul Lesbats

    Verified customer

    Submitted Aug 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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