Recombinant
RabMAb

Recombinant Anti-BANK1 antibody [EPR20795] - BSA and Azide free (ab230493)

Overview

  • Product name

    Anti-BANK1 antibody [EPR20795] - BSA and Azide free
    See all BANK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20795] to BANK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BANK1 aa 550 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8NDB2

  • Positive control

    • IHC-P: Human large B cell lymphoma tissue.
  • General notes

    Ab230493 is the carrier-free version of ab229189. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab230493 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab230493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).
Flow Cyt Use at an assay dependent concentration.

Target

  • Relevance

    BANK1 is a B cell specific scaffold protein and LYN tyrosine kinase substrate that promotes tyrosine phosphorylation of inositol 1,4,5-trisphosphate receptors.
  • Database links

  • Alternative names

    • A530094C12Rik antibody
    • AI451642 antibody
    • AVIEF antibody
    • B cell scaffold protein with ankyrin repeats 1 antibody
    • BANK antibody
    • FLJ20706 antibody
    • FLJ34204 antibody
    see all

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) (left panel) and Daudi (human Burkitt's lymphoma cell line) (right panel) cell lines labeling BANK1 with ab229189 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229189).

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in rat spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229189).

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in mouse spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229189).

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229189).

  • Immunohistochemical analysis of paraffin-embedded human large B cell lymphoma tissue labeling BANK1 with ab229189 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Cytoplasmic staining in human large B cell lymphoma cells is observed (PMID: 24879116). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229189).

References

ab230493 has not yet been referenced specifically in any publications.

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