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Anti-BAP1 antibody (ab167250)

  • Datasheet
  • SDS
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Western blot - Anti-BAP1 antibody (ab167250)
  • Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)

Key features and details

  • Mouse polyclonal to BAP1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BAP1 antibody
    See all BAP1 primary antibodies
  • Description

    Mouse polyclonal to BAP1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • BAP1 transfected 293T cell lysate; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human BAP1 protein (ab152849)
  • Related Products

    • Recombinant Human BAP1 protein (ab152849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
  • Tissue specificity

    Highly expressed in testis, placenta and ovary. Expressed in breast.
  • Sequence similarities

    Belongs to the peptidase C12 family. BAP1 subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
  • Target information above from: UniProt accession Q92560 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8314 Human
    • Omim: 603089 Human
    • SwissProt: Q92560 Human
    • Unigene: 106674 Human
    • Alternative names

      • BAP 1 antibody
      • Bap1 antibody
      • BAP1_HUMAN antibody
      • BRCA 1 associated protein 1 antibody
      • BRCA1 associated protein 1 antibody
      • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antibody
      • BRCA1-associated protein 1 antibody
      • Cerebral protein 13 antibody
      • Cerebral protein 6 antibody
      • DKFZp686N04275 antibody
      • FLJ35406 antibody
      • FLJ37180 antibody
      • HUCEP 13 antibody
      • Hucep 6 antibody
      • HUCEP13 antibody
      • Hucep6 antibody
      • KIAA0272 antibody
      • TPDS antibody
      • Ubiquitin carboxy terminal hydrolase antibody
      • Ubiquitin carboxyl terminal hydrolase BAP 1 antibody
      • Ubiquitin carboxyl terminal hydrolase BAP1 antibody
      • Ubiquitin carboxyl-terminal hydrolase BAP1 antibody
      • UCHL2 antibody
      see all

    Images

    • Western blot - Anti-BAP1 antibody (ab167250)
      Western blot - Anti-BAP1 antibody (ab167250)
      All lanes : Anti-BAP1 antibody (ab167250) at 1 µg

      Lane 1 : BAP1 transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 15 µl per lane.

      Developed using the ECL technique.

      Predicted band size: 80 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)
      Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)
      Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab167250? Please let us know so that we can cite the reference in this datasheet.

    ab167250 has been referenced in 1 publication.

    • Sahtoe DD  et al. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun 7:10292 (2016). PubMed: 26739236

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