Overview

  • Product name
  • Description
    Mouse polyclonal to BAP1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).

  • Positive control
    • BAP1 transfected 293T cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab167250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
  • Tissue specificity
    Highly expressed in testis, placenta and ovary. Expressed in breast.
  • Sequence similarities
    Belongs to the peptidase C12 family. BAP1 subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAP 1 antibody
    • Bap1 antibody
    • BAP1_HUMAN antibody
    • BRCA 1 associated protein 1 antibody
    • BRCA1 associated protein 1 antibody
    • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antibody
    • BRCA1-associated protein 1 antibody
    • Cerebral protein 13 antibody
    • Cerebral protein 6 antibody
    • DKFZp686N04275 antibody
    • FLJ35406 antibody
    • FLJ37180 antibody
    • HUCEP 13 antibody
    • Hucep 6 antibody
    • HUCEP13 antibody
    • Hucep6 antibody
    • KIAA0272 antibody
    • TPDS antibody
    • Ubiquitin carboxy terminal hydrolase antibody
    • Ubiquitin carboxyl terminal hydrolase BAP 1 antibody
    • Ubiquitin carboxyl terminal hydrolase BAP1 antibody
    • Ubiquitin carboxyl-terminal hydrolase BAP1 antibody
    • UCHL2 antibody
    see all

Images

  • All lanes : Anti-BAP1 antibody (ab167250) at 1 µg

    Lane 1 : BAP1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 15 µl per lane.

    Developed using the ECL technique.

    Predicted band size: 80 kDa

  • Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.

References

This product has been referenced in:
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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