Anti-BAP1 antibody (ab167250)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-BAP1 antibody
See all BAP1 primary antibodies -
DescriptionMouse polyclonal to BAP1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).
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Positive control
- BAP1 transfected 293T cell lysate; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab167250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionDeubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
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Tissue specificityHighly expressed in testis, placenta and ovary. Expressed in breast.
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Sequence similaritiesBelongs to the peptidase C12 family. BAP1 subfamily.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
- Information by UniProt
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Database links
- Entrez Gene: 8314 Human
- Omim: 603089 Human
- SwissProt: Q92560 Human
- Unigene: 106674 Human
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Alternative names
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
Images
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Western blot - Anti-BAP1 antibody (ab167250)All lanes : Anti-BAP1 antibody (ab167250) at 1 µg
Lane 1 : BAP1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.
Datasheets and documents
References
This product has been referenced in:
- Sahtoe DD et al. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun 7:10292 (2016). Read more (PubMed: 26739236) »
