Anti-BAP1 antibody (ab167250)
Key features and details
- Mouse polyclonal to BAP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BAP1 antibody
See all BAP1 primary antibodies -
Description
Mouse polyclonal to BAP1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).
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Positive control
- BAP1 transfected 293T cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. -
Tissue specificity
Highly expressed in testis, placenta and ovary. Expressed in breast. -
Sequence similarities
Belongs to the peptidase C12 family. BAP1 subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin. - Information by UniProt
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Database links
- Entrez Gene: 8314 Human
- Omim: 603089 Human
- SwissProt: Q92560 Human
- Unigene: 106674 Human
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Alternative names
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
Images
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Western blot - Anti-BAP1 antibody (ab167250)All lanes : Anti-BAP1 antibody (ab167250) at 1 µg
Lane 1 : BAP1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-BAP1 antibody (ab167250)Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab167250 has been referenced in 1 publication.
- Sahtoe DD et al. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun 7:10292 (2016). PubMed: 26739236