Overview

  • Product name

  • Description

    Rabbit polyclonal to BAP1
  • Host species

    Rabbit
  • Specificity

    ab199396 detects endogenous level of total BAP1 protein.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Fusion protein within Human BAP1 (internal sequence). The exact sequence is proprietary. Fusion protein corresponding to a region derived from internal residues of human BAP1. NCBI Accession No. BC001596. The identity of the protein fusion partner is GST.
    Database link: Q92560

  • Positive control

    • WB: HeLa whole cell lysate (ab150035). IHC-P: Human ovarian cancer tissue. Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab199396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/375 - 1/2000. Predicted molecular weight: 80 kDa.

Target

  • Function

    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
  • Tissue specificity

    Highly expressed in testis, placenta and ovary. Expressed in breast.
  • Sequence similarities

    Belongs to the peptidase C12 family. BAP1 subfamily.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
  • Information by UniProt
  • Database links

  • Alternative names

    • BAP 1 antibody
    • Bap1 antibody
    • BAP1_HUMAN antibody
    • BRCA 1 associated protein 1 antibody
    • BRCA1 associated protein 1 antibody
    • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antibody
    • BRCA1-associated protein 1 antibody
    • Cerebral protein 13 antibody
    • Cerebral protein 6 antibody
    • DKFZp686N04275 antibody
    • FLJ35406 antibody
    • FLJ37180 antibody
    • HUCEP 13 antibody
    • Hucep 6 antibody
    • HUCEP13 antibody
    • Hucep6 antibody
    • KIAA0272 antibody
    • TPDS antibody
    • Ubiquitin carboxy terminal hydrolase antibody
    • Ubiquitin carboxyl terminal hydrolase BAP 1 antibody
    • Ubiquitin carboxyl terminal hydrolase BAP1 antibody
    • Ubiquitin carboxyl-terminal hydrolase BAP1 antibody
    • UCHL2 antibody
    see all

Images

  • Anti-BAP1 antibody (ab199396) at 1/375 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 40 µg

    Predicted band size: 80 kDa



    10% SDS-PAGE

  • ab199396 staining BAP1 in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Ventana blocking agent for 4 minutes at 36°C; antigen retrieval was by heat mediation in EDTA pH 8.5. Samples were incubated with primary antibody (1/50 in blocking buffer) for 32 minutes at 37°C. An undiluted HRP polymer-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling BAP1 using ab199396 at a 1/40 dilution.

References

This product has been referenced in:

  • Wu J  et al. An Integrated Score and Nomogram Combining Clinical and Immunohistochemistry Factors to Predict High ISUP Grade Clear Cell Renal Cell Carcinoma. Front Oncol 8:634 (2018). Read more (PubMed: 30619768) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization
No
Specification
Testis
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 01 2015

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