Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization
No
Specification
Testis
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Other product details
Incubation time
32 minute(s) · Temperature: 37°C · Diluent: Ventana antibody diluent
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit Optiview detection system
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Used Ventana Benchmark Ultra automated platform, with Optiview detection kit.
Antigen retrieval was for 32 minutes at 100C.
Photo shown is of testis control tissue used to optimise the antibody.
Also succesfully tested in lung - no image available
Abcam user community
Verified customer
Submitted Sep 01 2015