Overview

  • Product name

  • Description

    Rabbit polyclonal to BAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide within Human BAP1 aa 500-550. The exact sequence is proprietary. NP_004647.1
    Database link: Q92560

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
    • Tissue specificity

      Highly expressed in testis, placenta and ovary. Expressed in breast.
    • Sequence similarities

      Belongs to the peptidase C12 family. BAP1 subfamily.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
    • Information by UniProt
    • Database links

    • Alternative names

      • BAP 1 antibody
      • Bap1 antibody
      • BAP1_HUMAN antibody
      • BRCA 1 associated protein 1 antibody
      • BRCA1 associated protein 1 antibody
      • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antibody
      • BRCA1-associated protein 1 antibody
      • Cerebral protein 13 antibody
      • Cerebral protein 6 antibody
      • DKFZp686N04275 antibody
      • FLJ35406 antibody
      • FLJ37180 antibody
      • HUCEP 13 antibody
      • Hucep 6 antibody
      • HUCEP13 antibody
      • Hucep6 antibody
      • KIAA0272 antibody
      • TPDS antibody
      • Ubiquitin carboxy terminal hydrolase antibody
      • Ubiquitin carboxyl terminal hydrolase BAP 1 antibody
      • Ubiquitin carboxyl terminal hydrolase BAP1 antibody
      • Ubiquitin carboxyl-terminal hydrolase BAP1 antibody
      • UCHL2 antibody
      see all

    Images

    • BAP1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

      ab245390 used for IP at 10 µg/mg lysate. For WB another anti-BAP1 antibody was used at 1 µg/ml.

      Lane 1: ab245390 IP in HeLa whole cell lysate.
      Lane 2: Control IgG.

      Chemiluminescence detection: 30 seconds.

    References

    ab245390 has not yet been referenced specifically in any publications.

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