Key features and details
- Rabbit polyclonal to BAP1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BAP1 antibody
See all BAP1 primary antibodies
DescriptionRabbit polyclonal to BAP1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
Synthetic peptide within Human BAP1 aa 500-550. The exact sequence is proprietary. NP_004647.1
Database link: Q92560
- IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245390 was affinity purified using an epitope specific to BAP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
FunctionDeubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.
Tissue specificityHighly expressed in testis, placenta and ovary. Expressed in breast.
Sequence similaritiesBelongs to the peptidase C12 family. BAP1 subfamily.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
- Information by UniProt
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
BAP1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245390 used for IP at 10 µg/mg lysate. For WB another anti-BAP1 antibody was used at 1 µg/ml.
Lane 1: ab245390 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245390 has not yet been referenced specifically in any publications.