RecombinantRabMAb

Recombinant Anti-BAP1 antibody [EPR22826-65] (ab255611)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
  • Anti-BAP1 antibody [EPR22826-65] (ab255611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22826-65] to BAP1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-BAP1 antibody [EPR22826-65]
    See all BAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22826-65] to BAP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, PC-3, HepG2, A375, MDA-MB-231 and A549 whole cell lysates. Flow Cyt (intra): PC-3 cells. IHC: Rat and mouse cerebrum, Human lung and colon carcinoma.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab255611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100.
Notes
Flow Cyt (Intra)
1/60.
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/100.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.

  • Tissue specificity

    Highly expressed in testis, placenta and ovary. Expressed in breast.

  • Sequence similarities

    Belongs to the peptidase C12 family. BAP1 subfamily.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin.
  • Information by UniProt

  • Database links

    • Alternative names

      • BAP 1 antibody
      • Bap1 antibody
      • BAP1_HUMAN antibody
      • BRCA 1 associated protein 1 antibody
      • BRCA1 associated protein 1 antibody
      • BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) antibody
      • BRCA1-associated protein 1 antibody
      • Cerebral protein 13 antibody
      • Cerebral protein 6 antibody
      • DKFZp686N04275 antibody
      • FLJ35406 antibody
      • FLJ37180 antibody
      • HUCEP 13 antibody
      • Hucep 6 antibody
      • HUCEP13 antibody
      • Hucep6 antibody
      • KIAA0272 antibody
      • TPDS antibody
      • Ubiquitin carboxy terminal hydrolase antibody
      • Ubiquitin carboxyl terminal hydrolase BAP 1 antibody
      • Ubiquitin carboxyl terminal hydrolase BAP1 antibody
      • Ubiquitin carboxyl-terminal hydrolase BAP1 antibody
      • UCHL2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on rat cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on mouse cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human lung carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody.  Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human colon carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    • Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      All lanes : Anti-BAP1 antibody [EPR22826-65] (ab255611) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
      Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 4 : A375 (human malignant melanoma epithelial cell), whole cell lysate
      Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 80 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      The molecular weight observed is consistent with what has been described in the literature (PMID: 18757409).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling BAP1 with ab255611 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Anti-BAP1 antibody [EPR22826-65] (ab255611)
      Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab255611? Please let us know so that we can cite the reference in this datasheet.

    ab255611 has not yet been referenced specifically in any publications.

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