Recombinant Anti-BAP1 antibody [EPR22826-65] (ab255611)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-454537-AntiBAP1-antibody-IHC.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22826-65] to BAP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-BAP1 antibody [EPR22826-65]
See all BAP1 primary antibodies -
Description
Rabbit monoclonal [EPR22826-65] to BAP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, PC-3, HepG2, A375, MDA-MB-231 and A549 whole cell lysates. Flow Cyt (intra): PC-3 cells. IHC: Rat and mouse cerebrum, Human lung and colon carcinoma.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22826-65 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/60.
|
|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).
|
|
| IHC-P |
1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/60. |
|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa). |
|
IHC-P
1/100. |
Target
-
Function
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. -
Tissue specificity
Highly expressed in testis, placenta and ovary. Expressed in breast. -
Sequence similarities
Belongs to the peptidase C12 family. BAP1 subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin. - Information by UniProt
-
Database links
- Entrez Gene: 8314 Human
- Entrez Gene: 104416 Mouse
- Entrez Gene: 306257 Rat
- Omim: 603089 Human
- SwissProt: Q92560 Human
- SwissProt: Q99PU7 Mouse
- SwissProt: D3ZHS6 Rat
- Unigene: 106674 Human
see all -
Alternative names
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on rat cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-454534-AntiBAP1-antibody-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on mouse cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-454532-AntiBAP1-antibody-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human lung carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-454530-AntiBAP1-antibody-IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human colon carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument -
![Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-352749-WB1anti-bap1-antibody-epr22826-65-western-blot-human.jpg)
Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)All lanes : Anti-BAP1 antibody [EPR22826-65] (ab255611) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 4 : A375 (human malignant melanoma epithelial cell), whole cell lysate
Lane 5 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe molecular weight observed is consistent with what has been described in the literature (PMID: 18757409).
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-1-FC1anti-bap1-antibody-epr22826-65-flowcytometry-pc3-human.jpg)
Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling BAP1 with ab255611 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Anti-BAP1 antibody [EPR22826-65] (ab255611)](https://www.abcam.com/ps/products/255/ab255611/Images/ab255611-3-benefits-of-recombinant-antibodies.png)
Anti-BAP1 antibody [EPR22826-65] (ab255611)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab255611 has not yet been referenced specifically in any publications.
