Recombinant Anti-BAP29 antibody [EPR7781] (ab129023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7781] to BAP29
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BAP29 antibody [EPR7781]
See all BAP29 primary antibodies -
Description
Rabbit monoclonal [EPR7781] to BAP29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BAP29 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- Fetal brain, U87-MG, fetal heart, Jurkat, SW480, HeLa lysates. Human brain tissue. HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.90 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7781 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. -
Sequence similarities
Belongs to the BCAP29/BCAP31 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 55973 Human
- GenBank: NP_001008405 Human
- SwissProt: Q9UHQ4 Human
- Unigene: 303787 Human
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Alternative names
- B cell receptor associated protein 29 antibody
- B-cell receptor-associated protein 29 antibody
- Bap29 antibody
see all
Images
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All lanes : Anti-BAP29 antibody [EPR7781] (ab129023) at 1/10000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Fetal heart tissue lysate
Lane 4 : Jurkat cell lysate
Lane 5 : SW480 cell lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa -
ab129023, at 1/100 dilution staining BAP29 in formalin fixed paraffin embedded Human brain tissue by immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab129023, at 1/100, staining BAP29 in Hela cells by immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129023 has not yet been referenced specifically in any publications.