Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7781] to BAP29
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-BAP29 antibody [EPR7781]
See all BAP29 primary antibodies
DescriptionRabbit monoclonal [EPR7781] to BAP29
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human BAP29 aa 200-300 (C terminal). The exact sequence is proprietary.
- Fetal brain, U87-MG, fetal heart, Jurkat, SW480, HeLa lysates. Human brain tissue. HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.90 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
RelevanceBAP29 and the related protein BAP31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both BAP29 and BAP31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the BAP29/BAP31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that BAP29 and BAP31 may act as chaperones for transmembrane regions of various proteins. There are multiple isoforms of BAP29.
Cellular localizationEndoplasmic reticulum
- B cell receptor associated protein 29 antibody
- BCAP29 antibody
- BCR associated protein 29 antibody
All lanes : Anti-BAP29 antibody [EPR7781] (ab129023) at 1/10000 dilution
Lane 1 : Fetal brain cell lysates
Lane 2 : U87-MG
Lane 3 : Fetal heart cell lysates
Lane 4 : Jurkat cell lysates
Lane 5 : SW480 cell lysates
Lane 6 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 27 kDa
ab129023, at 1/100 dilution staining BAP29 in formalin fixed paraffin embedded Human brain tissue by immunohistochemistry
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab129023, at 1/100, staining BAP29 in Hela cells by immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129023 has not yet been referenced specifically in any publications.