Recombinant
RabMAb

Recombinant Anti-BAP29 antibody [EPR7781] (ab129023)

Overview

  • Product name

    Anti-BAP29 antibody [EPR7781]
    See all BAP29 primary antibodies
  • Description

    Rabbit monoclonal [EPR7781] to BAP29
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BAP29 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain, U87-MG, fetal heart, Jurkat, SW480, HeLa lysates. Human brain tissue. HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      BAP29 and the related protein BAP31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both BAP29 and BAP31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the BAP29/BAP31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that BAP29 and BAP31 may act as chaperones for transmembrane regions of various proteins. There are multiple isoforms of BAP29.
    • Cellular localization

      Endoplasmic reticulum
    • Database links

    • Alternative names

      • B cell receptor associated protein 29 antibody
      • BCAP29 antibody
      • BCR associated protein 29 antibody

    Images

    • All lanes : Anti-BAP29 antibody [EPR7781] (ab129023) at 1/10000 dilution

      Lane 1 : Fetal brain cell lysates
      Lane 2 : U87-MG
      Lane 3 : Fetal heart cell lysates
      Lane 4 : Jurkat cell lysates
      Lane 5 : SW480 cell lysates
      Lane 6 : HeLa cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 27 kDa

    • ab129023, at 1/100 dilution staining BAP29 in formalin fixed paraffin embedded Human brain tissue by immunohistochemistry
    • ab129023, at 1/100, staining BAP29 in Hela cells by immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab129023 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129023.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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