Product nameBAP29 overexpression 293T lysate (whole cell)
ab94232 is a 293T cell transfected lysate in which Human BAP29 has been transiently over-expressed using a pCMV-BAP29 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundBAP29 and the related protein BAP31 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Both BAP29 and BAP31 interact with membrane-bound immunoglobulins (mIgs), such as IgM and IgD, which with Ig-alpha/Ig-beta heterodimers form B cell antigen receptors. Binding of the BAP29/BAP31 heterodimer correlates with the ER retention of non-Ig-alpha/Ig-beta bound mIg complexes, suggesting that BAP29 and BAP31 may act as chaperones for transmembrane regions of various proteins. There are multiple isoforms of BAP29.
Our Abpromise guarantee covers the use of ab94232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94232 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-BAP29 antibody (ab57493) at 1/500 dilution
Lane 1 :
BAP29 overexpression 293T lysate (whole cell) (ab94232)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94232 has not yet been referenced specifically in any publications.