Anti-BAP31 antibody (ab10924)
Key features and details
- Goat polyclonal to BAP31
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-BAP31 antibody
See all BAP31 primary antibodies -
Description
Goat polyclonal to BAP31 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide: QAAVDGPMDKKEE, corresponding to C terminal amino acids 234-246 of Human BCAP31.
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Positive control
- WB: Human placenta and brain lysate. IHC-P: Human Kidney, Cerebellum, and Cortex tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 3.75 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1. -
Sequence similarities
Belongs to the BCAP29/BCAP31 family. -
Post-translational
modificationsCleaved by CASP8 and other caspases. -
Cellular localization
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex. - Information by UniProt
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Database links
- Entrez Gene: 10134 Human
- Omim: 300398 Human
- SwissProt: P51572 Human
- Unigene: 522817 Human
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Alternative names
- 6C6 AG antibody
- 6C6 AG tumor associated antigen antibody
- 6C6-AG tumor-associated antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-BAP31 antibody (ab10924)Anti-BAP31 antibody (ab10924) at 0.1 µg/ml + Human breast lysate (in RIPA buffer) at 35 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Datasheets and documents
References (0)
ab10924 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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