Anti-BAP31 antibody (ab10924)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-BAP31 antibody
See all BAP31 primary antibodies -
DescriptionGoat polyclonal to BAP31
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Host speciesGoat
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Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide: QAAVDGPMDKKEE, corresponding to C terminal amino acids 234-246 of Human BCAP31.
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Positive control
- WB: Human placenta and brain lysate. IHC-P: Human Kidney, Cerebellum, and Cortex tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab10924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/32000. This antibody has only been tested against the recombinant protein. | |
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).Can be blocked with Human BAP31 peptide (ab23203). | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
Target
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FunctionMay play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
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Tissue specificityUbiquitous.
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Involvement in diseaseNote=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
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Sequence similaritiesBelongs to the BCAP29/BCAP31 family.
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Post-translational
modificationsCleaved by CASP8 and other caspases. -
Cellular localizationEndoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
- Information by UniProt
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Database links
- Entrez Gene: 10134 Human
- Omim: 300398 Human
- SwissProt: P51572 Human
- Unigene: 522817 Human
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Alternative names
- 6C6 AG antibody
- 6C6 AG tumor associated antigen antibody
- 6C6-AG tumor-associated antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-BAP31 antibody (ab10924)Anti-BAP31 antibody (ab10924) at 0.1 µg/ml + Human breast lysate (in RIPA buffer) at 35 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Protocols
Datasheets and documents
References
ab10924 has not yet been referenced specifically in any publications.
