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    bap31-antibody-ab10924.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-BAP31 antibody (ab10924)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
  • Western blot - Anti-BAP31 antibody (ab10924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)

Key features and details

  • Goat polyclonal to BAP31
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-BAP31 antibody [EPR3878(2)] (ab109304)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-BAP31 antibody
    See all BAP31 primary antibodies
  • Description

    Goat polyclonal to BAP31
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide: QAAVDGPMDKKEE, corresponding to C terminal amino acids 234-246 of Human BCAP31.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human placenta and brain lysate. IHC-P: Human Kidney, Cerebellum, and Cortex tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human BAP31 protein (ab132144)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

1 hour primary incubation is recommended for this product.

IHC-P
Use a concentration of 3.75 µg/ml.
Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

1 hour primary incubation is recommended for this product.

IHC-P
Use a concentration of 3.75 µg/ml.

Target

  • Function

    May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • Sequence similarities

    Belongs to the BCAP29/BCAP31 family.
  • Post-translational
    modifications

    Cleaved by CASP8 and other caspases.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Target information above from: UniProt accession P51572 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10134 Human
    • Omim: 300398 Human
    • SwissProt: P51572 Human
    • Unigene: 522817 Human
    • Alternative names

      • 6C6 AG antibody
      • 6C6 AG tumor associated antigen antibody
      • 6C6-AG tumor-associated antigen antibody
      • 6C6AG antibody
      • 6C6AG tumor associated antigen antibody
      • Accessory protein BAP 31 antibody
      • Accessory protein BAP31 antibody
      • B cell receptor associated protein 31 antibody
      • B-cell receptor-associated protein 31 antibody
      • BA31 antibody
      • BAP 31 antibody
      • Bap31 antibody
      • BAP31_HUMAN antibody
      • BCAP 31 antibody
      • BCAP31 antibody
      • BCR associated protein Bap 31 antibody
      • BCR associated protein Bap31 antibody
      • BCR-associated protein 31 antibody
      • CDM antibody
      • CDM protein antibody
      • DXS1357E antibody
      • MS950 antibody
      • p28 antibody
      • p28 Bap31 antibody
      • Protein CDM antibody
      • RP23-329M9.5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)

      ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    • Western blot - Anti-BAP31 antibody (ab10924)
      Western blot - Anti-BAP31 antibody (ab10924)
      Anti-BAP31 antibody (ab10924) at 0.1 µg/ml + Human breast lysate (in RIPA buffer) at 35 µg

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)

      ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab10924)

      ab10924 (3.75 µg/ml) staining BAP31 in paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab10924? Please let us know so that we can cite the reference in this datasheet.

    ab10924 has not yet been referenced specifically in any publications.

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