Overview

  • Product name

  • Description

    Rabbit polyclonal to BAP31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human BAP31 aa 2-243.
    Database link: P51572

  • Positive control

    • WB: HEK-293, HeLa, MCF7 and SH-SY5Y whole cell lysates; Mouse and rat liver and brain lysates. IHC-P: Human colon cancer and prostate cancer tissue. ICC/IF: HeLa cells. IP: Mouse liver lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
IP 1/200 - 1/2000.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

Target

  • Function

    May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • Sequence similarities

    Belongs to the BCAP29/BCAP31 family.
  • Post-translational
    modifications

    Cleaved by CASP8 and other caspases.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6C6 AG antibody
    • 6C6 AG tumor associated antigen antibody
    • 6C6-AG tumor-associated antigen antibody
    • 6C6AG antibody
    • 6C6AG tumor associated antigen antibody
    • Accessory protein BAP 31 antibody
    • Accessory protein BAP31 antibody
    • B cell receptor associated protein 31 antibody
    • B-cell receptor-associated protein 31 antibody
    • BA31 antibody
    • BAP 31 antibody
    • Bap31 antibody
    • BAP31_HUMAN antibody
    • BCAP 31 antibody
    • BCAP31 antibody
    • BCR associated protein Bap 31 antibody
    • BCR associated protein Bap31 antibody
    • BCR-associated protein 31 antibody
    • CDM antibody
    • CDM protein antibody
    • DXS1357E antibody
    • MS950 antibody
    • p28 antibody
    • p28 Bap31 antibody
    • Protein CDM antibody
    • RP23-329M9.5 antibody
    see all

Images

  • All lanes : Anti-BAP31 antibody (ab237485) at 1/1000 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat brain tissue lysate
    Lane 7 : Mouse liver tissue lysate
    Lane 8 : Mouse brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 28 kDa

  • Paraffin-embedded human colon cancer tissue stained for BAP31 using ab237485 at 1/1200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • Paraffin-embedded human prostate cancer tissue stained for BAP31 using ab237485 at 1/1200 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BAP31 (green) using ab237485 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

  • BAP31 was immunoprecipitated from mouse liver tissue lysate using ab237485 at 1/200 dilution.

    Lane 1: Rabbit control IgG in mouse liver tissue lysate.

    Lane 2: ab237485 in mouse liver tissue lysate.

    Lane 3: Mouse liver tissue lysate (10 µg input).

    For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.

References

ab237485 has not yet been referenced specifically in any publications.

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