Overview

  • Product name

  • Description

    Rabbit polyclonal to BAP31
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 17 amino acids near to the C terminus of the human BAP31 protein.

  • Positive control

    • Ramos cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: PC12.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).

Target

  • Function

    May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • Tissue specificity

    Ubiquitous.
  • Involvement in disease

    Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • Sequence similarities

    Belongs to the BCAP29/BCAP31 family.
  • Post-translational
    modifications

    Cleaved by CASP8 and other caspases.
  • Cellular localization

    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6C6 AG antibody
    • 6C6 AG tumor associated antigen antibody
    • 6C6-AG tumor-associated antigen antibody
    • 6C6AG antibody
    • 6C6AG tumor associated antigen antibody
    • Accessory protein BAP 31 antibody
    • Accessory protein BAP31 antibody
    • B cell receptor associated protein 31 antibody
    • B-cell receptor-associated protein 31 antibody
    • BA31 antibody
    • BAP 31 antibody
    • Bap31 antibody
    • BAP31_HUMAN antibody
    • BCAP 31 antibody
    • BCAP31 antibody
    • BCR associated protein Bap 31 antibody
    • BCR associated protein Bap31 antibody
    • BCR-associated protein 31 antibody
    • CDM antibody
    • CDM protein antibody
    • DXS1357E antibody
    • MS950 antibody
    • p28 antibody
    • p28 Bap31 antibody
    • Protein CDM antibody
    • RP23-329M9.5 antibody
    see all

Images

  • Lane 1 : Anti-BAP31 antibody (ab37120) at 0.5 µg/ml
    Lane 2 : Anti-BAP31 antibody (ab37120) at 1 µg/ml
    Lane 3 : Anti-BAP31 antibody (ab37120) at 2 µg/ml

    All lanes : Ramos cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 28 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?

  • ICC/IF image of ab37120 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37120 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab37120 at 2µg/ml staining BAP31 in rat lung tissue by IHC

References

This product has been referenced in:

  • Ma C  et al. Low expression of B-Cell-Associated protein 31 is associated with unfavorable prognosis in human colorectal cancer. Pathol Res Pract 214:661-666 (2018). Read more (PubMed: 29653744) »
  • Kim IY  et al. Loperamide overcomes the resistance of colon cancer cells to bortezomib by inducing CHOP-mediated paraptosis-like cell death. Biochem Pharmacol N/A:N/A (2018). Read more (PubMed: 30529689) »
See all 5 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
African Green Monkey Cell lysate - other (COS 7)
Loading amount
10 µg
Specification
COS 7
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Sep 28 2010

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