Application
Western blot
Sample
African Green Monkey Cell lysate - other (COS 7)
Loading amount
10 µg
Specification
COS 7
Gel Running Conditions
Reduced Denaturing (15 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS tween 0.05%
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit hrp
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 31 kDa
Additional data
Abcam response
Thank you for your Abreview.
Wavy bands maybe due to old gel. Diluting the antibody in the blocking buffer might reduce background in addition to using more diluted antibody.
Wavy bands maybe due to old gel. Diluting the antibody in the blocking buffer might reduce background in addition to using more diluted antibody.
Abcam user community
Verified customer
Submitted Sep 28 2010