Overview

  • Product name

  • Description

    Rabbit polyclonal to BAPX1
  • Host species

    Rabbit
  • Specificity

    ab133204 is predicted not to cross-react with other NKX homeobox proteins.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to an internal region of Human BAPX1.

  • Positive control

    • Human brain cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab133204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus.
  • Tissue specificity

    Expressed at highest levels in cartilage, bone (osteosarcoma) and gut (small intestine and colon), whereas moderate expression is seen in trachea and brain. Expressed in visceral mesoderm and embryonic skeleton.
  • Involvement in disease

    Defects in NKX3-2 are the cause of spondylo-megaepiphyseal-metaphyseal dysplasia (SMMD) [MIM:613330]. It is a skeletal dysplasia characterized by disproportionate short stature with a short and stiff neck and trunk, relatively long limbs that may show flexion contractures of the distal joints, delayed and impaired ossification of the vertebral bodies, the presence of large epiphysea ossification centers and wide growth plates in the long tubular bones and numerous pseudoepiphyses of the short tubular bones in hands and feet.
  • Sequence similarities

    Belongs to the NK-3 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bagpipe homeobox 1 antibody
    • Bagpipe homeobox homolog 1 antibody
    • Bagpipe homeobox protein homolog 1 antibody
    • Bagpipe homeobox, Drosophila, homolog of, 1 antibody
    • BAPX 1 antibody
    • Homeobox protein NK-3 homolog B antibody
    • Homeobox protein Nkx 3.2 antibody
    • Homeobox protein Nkx-3.2 antibody
    • MGC138171 antibody
    • NK3 homeobox 2 antibody
    • NKX3 2 antibody
    • NKX3-2 antibody
    • NKX3.2 antibody
    • NKX3.2, mouse, homolog of antibody
    • NKX32_HUMAN antibody
    • NKX3B antibody
    • OTTHUMP00000115816 antibody
    • SMMD antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain cortex tissue labelling BAPX1 with ab133204 at 5 µg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab133204 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (10mM Sodium Citrate, 0.05% Tween 20, pH 6.0)
Permeabilization
Yes - PBS-Tween 0.05%
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 18°C
Fixative
Paraformaldehyde

Miss. Nicola Smith

Verified customer

Submitted Jul 17 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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