Anti-BAPX1 antibody (ab83288)
Key features and details
- Rabbit polyclonal to BAPX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BAPX1 antibody
See all BAPX1 primary antibodies -
Description
Rabbit polyclonal to BAPX1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Saccharomyces cerevisiae
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Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (GAGGGGGSGP AGVAEEEEEP AAPKPRKKRS RAAFSHAQVF ELERRFNHQR) of human BAPX1 (NP_001180).
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Positive control
- WB: OVCAR-3 and 293T cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab83288 is purified by a peptide affinity chromatography method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
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| Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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Function
Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development; required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear; required for tympanic ring and gonium development and in the regulation of the width of the malleus. -
Tissue specificity
Expressed at highest levels in cartilage, bone (osteosarcoma) and gut (small intestine and colon), whereas moderate expression is seen in trachea and brain. Expressed in visceral mesoderm and embryonic skeleton. -
Involvement in disease
Defects in NKX3-2 are the cause of spondylo-megaepiphyseal-metaphyseal dysplasia (SMMD) [MIM:613330]. It is a skeletal dysplasia characterized by disproportionate short stature with a short and stiff neck and trunk, relatively long limbs that may show flexion contractures of the distal joints, delayed and impaired ossification of the vertebral bodies, the presence of large epiphysea ossification centers and wide growth plates in the long tubular bones and numerous pseudoepiphyses of the short tubular bones in hands and feet. -
Sequence similarities
Belongs to the NK-3 homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 579 Human
- Entrez Gene: 12020 Mouse
- Entrez Gene: 682329 Rat
- Omim: 602183 Human
- SwissProt: P78367 Human
- SwissProt: P97503 Mouse
- Unigene: 590927 Human
- Unigene: 2530 Mouse
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Alternative names
- Bagpipe homeobox 1 antibody
- Bagpipe homeobox homolog 1 antibody
- Bagpipe homeobox protein homolog 1 antibody
see all
Images
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Western blot - Anti-BAPX1 antibody (ab83288)Anti-BAPX1 antibody (ab83288) at 1 µg/ml + OVCAR-3 cell lysate at 10 µg
Predicted band size: 35 kDaWestern blot analysis of OVCAR-3 cell lysate labeling BAPX1 with ab83288.
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Western blot - Anti-BAPX1 antibody (ab83288)All lanes : Anti-BAPX1 antibody (ab83288) at 1 µg/ml
Lane 1 : Human stomach tumor lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 35 kDaWestern blot analysis labeling BAPX1 with ab83288.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab83288 has been referenced in 3 publications.
- Yano F et al. Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation. Sci Rep 9:7666 (2019). PubMed: 31113964
- Backer G et al. FAR2 is associated with kidney disease in mice and humans. Physiol Genomics 50:543-552 (2018). PubMed: 29652635
- Kamel G et al. Requirement for frzb and fzd7a in cranial neural crest convergence and extension mechanisms during zebrafish palate and jaw morphogenesis. Dev Biol 381:423-33 (2013). PubMed: 23806211