Anti-BARD1 antibody (ab226854)
Key features and details
- Rabbit polyclonal to BARD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-BARD1 antibody
See all BARD1 primary antibodies -
Description
Rabbit polyclonal to BARD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human BARD1 (internal sequence). The exact sequence is proprietary.
Database link: Q99728 -
Positive control
- WB: A431 whole cell and nuclear extracts. IHC-P: Mouse and rat brain tissues. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsProcessed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer. -
Cellular localization
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. - Information by UniProt
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Database links
- Entrez Gene: 580 Human
- Entrez Gene: 12021 Mouse
- Entrez Gene: 64557 Rat
- Omim: 601593 Human
- SwissProt: Q99728 Human
- SwissProt: O70445 Mouse
- SwissProt: Q9QZH2 Rat
- Unigene: 591642 Human
see all -
Alternative names
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
Images
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All lanes : Anti-BARD1 antibody (ab226854) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis
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Paraffin-embedded rat brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis
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4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BARD1 (green) using ab226854 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab226854 has been referenced in 4 publications.
- Gan C et al. USP25 inhibits DNA damage by stabilizing BARD1 protein in a house dust mite-induced asthmatic model in vitro and in vivo. Cell Biol Int 46:922-932 (2022). PubMed: 35143098
- Li W et al. A synergetic effect of BARD1 mutations on tumorigenesis. Nat Commun 12:1243 (2021). PubMed: 33623049
- Minten EV et al. SIRT2 promotes BRCA1-BARD1 heterodimerization through deacetylation. Cell Rep 34:108921 (2021). PubMed: 33789098
- Zhao Z et al. MT2A Promotes Oxaliplatin Resistance in Colorectal Cancer Cells. Cell Biochem Biophys 78:475-482 (2020). PubMed: 32638210