Key features and details
- Rabbit polyclonal to BARD1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-BARD1 antibody
See all BARD1 primary antibodies
DescriptionRabbit polyclonal to BARD1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human BARD1 (internal sequence). The exact sequence is proprietary.
Database link: Q99728
- WB: A431 whole cell and nuclear extracts. IHC-P: Mouse and rat brain tissues. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- DNA Damage Recognition
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab226854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 87 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionProbable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger.
modificationsProcessed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
Cellular localizationNucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
- Information by UniProt
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
All lanes : Anti-BARD1 antibody (ab226854) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 2 : A431 nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis
Paraffin-embedded rat brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BARD1 (green) using ab226854 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226854 has not yet been referenced specifically in any publications.