• Product name

  • Description

    Rabbit polyclonal to BARD1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human BARD1 (internal sequence). The exact sequence is proprietary.
    Database link: Q99728

  • Positive control

    • WB: A431 whole cell and nuclear extracts. IHC-P: Mouse and rat brain tissues. ICC/IF: A431 cells.



Our Abpromise guarantee covers the use of ab226854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 87 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.


  • Function

    Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • Post-translational

    Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • Cellular localization

    Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all


  • All lanes : Anti-BARD1 antibody (ab226854) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 2 : A431 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 87 kDa

    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis

  • Paraffin-embedded rat brain tissue stained for BARD1 with ab226854 at 1/400 dilution in immunohistochemical analysis

  • 4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BARD1 (green) using ab226854 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.


ab226854 has not yet been referenced specifically in any publications.

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