Anti-BARD1 antibody (ab245434)
Key features and details
- Rabbit polyclonal to BARD1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BARD1 antibody
See all BARD1 primary antibodies -
Description
Rabbit polyclonal to BARD1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BARD1 aa 250-300. The exact sequence is proprietary. NP_000456.1
Database link: Q99728 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: BARD1 IP in HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245434 was affinity purified using an epitope specific to BARD1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/500 - 1/2500.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/500 - 1/2500. |
Target
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Function
Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 4 ANK repeats.
Contains 2 BRCT domains.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsProcessed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer. -
Cellular localization
Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. - Information by UniProt
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Database links
- Entrez Gene: 580 Human
- Omim: 601593 Human
- SwissProt: Q99728 Human
- Unigene: 591642 Human
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Alternative names
- BARD-1 antibody
- Bard1 antibody
- BARD1_HUMAN antibody
see all
Images
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All lanes : Anti-BARD1 antibody (ab245434) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds -
BARD1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245434 used for IP at 6 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245434 (Lot 2) IP in HeLa whole cell lysate.
Lane 2: ab245434 (Lot 3) IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245434 has not yet been referenced specifically in any publications.