Overview

  • Product name
  • Description
    Rabbit polyclonal to BarX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chicken, Cow, DogDoes not react with: Mouse
  • Immunogen

    Synthetic peptide within Human BarX1 aa 91-140 (internal sequence). The exact sequence is proprietary.
    Sequence:

    PLGCSGLSSALLAAGPGLPGAAGAPHLPLELQLRGKLEAAGPGEPGTKAK


    Database link: Q9HBU1

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    BarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • BarH like homeobox 1 antibody
    • BarX 1 antibody
    • BARX homeobox 1 antibody
    • Homeobox protein BarH like 1 antibody

Images

  • Anti-BarX1 antibody (ab26156) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size: 24 kDa

  • Paraffin embeded Tissue: Human Heart
    Cells with positive label: Myocardial cells (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X
  • Paraffin embeded Tissue: Human Pancreas
    Cells with positive label: Epithelial cells of pancreatic acinus (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X

References

This product has been referenced in:
  • Yan C  et al. An esophageal adenocarcinoma susceptibility locus at 9q22 also confers risk to esophageal squamous cell carcinoma by regulating the function of BARX1. Cancer Lett 421:103-111 (2018). IHC . Read more (PubMed: 29454095) »
  • Krivicka-Uzkurele B  et al. Barx1, growth factors and apoptosis in facial tissue of children with clefts. Stomatologija 10:62-6 (2008). IHC ; Human . Read more (PubMed: 18708738) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tooth)
Antigen retrieval step
None
Permeabilization
No
Specification
tooth
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 19 2015

Answer

Thank you for your reply. It is unfortunate that the sequence information for pig is not available. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for rewards such as Amazon.com gift certificates or discounts on future antibody purchases. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in pig. All tested species are listed on Abcam product datasheets. I am, however, enquiring with the originator of this product to find out the homology between the immunogen peptide sequence and that in pig. This will give you a better idea of whether we can expect this antibody to cross-react. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, I will be in contact as soon as I receive more information.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mandible, digestive tract, stomach)
Loading amount
30 µg
Specification
mandible, digestive tract, stomach
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Isabelle Miletich

Verified customer

Submitted Oct 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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