Overview

  • Product name

    Anti-BarX1 antibody [EPR14120]
    See all BarX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14120] to BarX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BarX1 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9HBU1

  • Positive control

    • WB: HepG2
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).

Target

  • Relevance

    BarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • BarH like homeobox 1 antibody
    • BarX 1 antibody
    • BARX homeobox 1 antibody
    • Homeobox protein BarH like 1 antibody

Images

  • All lanes : Anti-BarX1 antibody [EPR14120] (ab181851) at 1/1000 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) nuclear fraction lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) without nuclear fraction lysate
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 27 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Diluting/Blocking buffer and concentration: 5% NFDM /TBST

References

This product has been referenced in:

  • Yan C  et al. An esophageal adenocarcinoma susceptibility locus at 9q22 also confers risk to esophageal squamous cell carcinoma by regulating the function of BARX1. Cancer Lett 421:103-111 (2018). WB . Read more (PubMed: 29454095) »
  • Guo S  et al. GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1. Cell Death Differ N/A:N/A (2018). Read more (PubMed: 29523871) »
See all 3 Publications for this product

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