Product nameAnti-BarX1 antibody [EPR14120]
See all BarX1 primary antibodies
DescriptionRabbit monoclonal [EPR14120] to BarX1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human BarX1 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q9HBU1
- WB: HepG2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).|
RelevanceBarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development.
- BarH like homeobox 1 antibody
- BarX 1 antibody
- BARX homeobox 1 antibody
- Homeobox protein BarH like 1 antibody
All lanes : Anti-BarX1 antibody [EPR14120] (ab181851) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) nuclear fraction lysate
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) without nuclear fraction lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds
Diluting/Blocking buffer and concentration: 5% NFDM /TBST
This product has been referenced in:
- Yan C et al. An esophageal adenocarcinoma susceptibility locus at 9q22 also confers risk to esophageal squamous cell carcinoma by regulating the function of BARX1. Cancer Lett 421:103-111 (2018). WB . Read more (PubMed: 29454095) »
- Guo S et al. GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1. Cell Death Differ N/A:N/A (2018). Read more (PubMed: 29523871) »