Key features and details
- Rabbit polyclonal to BAS1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-BAS1 antibody
DescriptionRabbit polyclonal to BAS1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Arabidopsis thaliana, Triticum aestivum, Hordeum vulgare, Spinacia oleracea
Recombinant full length protein corresponding to Arabidopsis thaliana BAS1 aa 66-266.
KAQADDLPLVGNKAPDFEAEAVFDQEFIKVKLSDYIGKKYVILFFYPLDF TFVCPTEITAFSDRHSEFEKLNTEVLGVSVDSVFSHLAWVQTDRKSGGLG DLNYPLISDVTKSISKSFGVLIHDQGIALRGLFIIDKEGVIQHSTINNLG IGRSVDETMRTLQALQYIQENPDEVCPAGWKPGEKSMKPDPKLSKEYFSA I
Database link: Q96291
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab224746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.
This antibody has only been tested in WB using the recombinant protein, we have no data for endogenous protein.
FunctionMay be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf. Involved in the detoxification of alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system.
Sequence similaritiesBelongs to the AhpC/TSA family.
Contains 1 thioredoxin domain.
modificationsThe Cys-119-SH group is the primary site of oxidation by H(2)O(2), and the oxidized Cys-119 (probably Cys-SOH) rapidly reacts with Cys-241-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin.
Cellular localizationPlastid, chloroplast.
- Information by UniProt
- 2-Cys peroxiredoxin A antibody
- 2-Cys peroxiredoxin BAS1 antibody
- 2-Cys Prx A antibody
All lanes : Anti-BAS1 antibody (ab224746) at 1/500 dilution
Lane 1 : 60 ng recombinant protein
Lane 2 : 30 ng recombinant protein
Lane 3 : 15 ng recombinant protein
Lane 4 : 7.5 ng recombinant protein
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Developed using the ECL technique.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224746 has not yet been referenced specifically in any publications.