Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-basal cell Cytokeratin antibody [RCK103]
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DescriptionMouse monoclonal [RCK103] to basal cell Cytokeratin
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Host speciesMouse
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SpecificityThis antibody recognizes (amongst others) cytokeratin 5. It stains basal cells in combined and stratified epithelial tissues. It also recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
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Tested applicationsSuitable for: Dot blot, Flow Cyt, IHC-Fr, ICC, WBmore details
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Species reactivityReacts with: Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Dog, Human, Pig, Quail
Does not react with: Zebrafish -
Immunogen
Mix of cell preparations containing human cytokeratins.
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General notes
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein G purified
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Primary antibody notesCytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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ClonalityMonoclonal
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Clone numberRCK103
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab9222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Dot blot | 1/100 - 1/1000. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | 1/100 - 1/200. | |
| ICC | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/1000. |
Images
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![Immunohistochemistry (Frozen sections) - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)](https://www.abcam.com/ps/products/9/ab9222/Images/basal-cell-Cytokeratin-Primary-antibodies-ab9222-1.jpg)
Immunohistochemistry (Frozen sections) - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)Immunohistochemistry on frozen sections of human skin -
![Flow Cytometry - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)](https://www.abcam.com/ps/products/9/ab9222/Images/basal-cell-Cytokeratin-Primary-antibodies-ab9222-2.jpg)
Flow Cytometry - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)Overlay histogram showing A431 cells stained with ab9222 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9222, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunohistochemistry (Frozen sections) - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)](https://www.abcam.com/ps/products/9/ab9222/Images/basal-cell-Cytokeratin-Primary-antibodies-ab9222-3.jpg)
Immunohistochemistry (Frozen sections) - Anti-basal cell Cytokeratin antibody [RCK103] (ab9222)Immunofluorescence staining of a 7 days old zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs
Protocols
References
This product has been referenced in:
- Tamberg N et al. Keap1-MCM3 interaction is a potential coordinator of molecular machineries of antioxidant response and genomic DNA replication in metazoa. Sci Rep 8:12136 (2018). Read more (PubMed: 30108253) »
- Kuzinski J et al. Modulation of vH+-ATPase is part of the functional adaptation of sheep rumen epithelium to high-energy diet. Am J Physiol Regul Integr Comp Physiol 303:R909-20 (2012). Flow Cyt ; Sheep . Read more (PubMed: 22972839) »
