Product nameBasement Membrane Extract Kit for 3D Cell Culture
See all 3D Cell Culture kits
Assay typeCell-based (qualitative)
Basement Membrane Extract Kit for 3D Cell Culture (ab241012) offers 3D cell culture matrices to meet the needs and requirements of various research fields. This kits provide a standardized, yet user friendly and adaptable to high-throughput strategy for setting up spheroid formations, 3D cell cultures and pharmacological studies.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests BME Matrix 1 x 5ml Wash Buffer 1 x 100ml
RelevanceThree dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell cultures improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell cultures. 3D matrices provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies.
HEPG2 and MCF7 cells in No Matrix (A) and 3D BME Matrix (B). Cells were cultured in BME Matrix for 21 days, and successfully formed spheroids. Media was changed every 2-3 days as per protocol. The Calcein AM staining (C) indicates that cell viability is not affected while culturing in matrix for a long period of time. Note: Calcein AM is not included.
ab241012 has not yet been referenced specifically in any publications.