Product nameBaseMuncher Endonuclease
BaseMuncher Endonuclease (ab270049) is a non-specific endonuclease that hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’- phosphorylated oligonucleotides of 1-4 bases in length. Recombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27 kDa subunits that has exceptionally high specific activity and is completely free of protease activity. BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation/analysis, replacing crude DNase I in many applications.
This product is manufactured by Expedeon, an Abcam company. Expedeon product code BM0025 was previously called BaseMuncher Endonuclease >99% Ultrapure - 25,000 Units (1 x 100µl) and is the same as the 25,000 unit size of this product. Expedeon product code BM0100 was previously called BaseMuncher Endonuclease >99% Ultrapure - 100,000 Units (4 x 100µl) and is is the same as the 100,000 unit size.
Features & Benefits:
- Exceptionally high endonuclease specificity for DNA and RNA
- Ultrapure to >99%
- Free of protease activity
- High purity and high concentration grade
- Easy protein purification –Reduced viscosity during sample preparation
- 100x greater activity than DNAase
- Cheaper than Benzonase; but the same quality and efficiency.
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferConstituents: 50% Glycerol, 0.1% Magnesium chloride hexahydrate, 0.6% Tris, 0.3% Sodium chloride, 45% Water, 0.025% Enzyme
Concentration information loading...
BaseMuncher Endonuclease is purified by a proprietary process to be >99% pure by SDS-PAGE.
50 mg of salmon sperm DNA was incubated with the indicated units of BaseMuncher Endonuclease and another brand of nuclease at 37°C for 30 mins in a buffer containing 50 mM Tris-HCl; pH 8.0 and 1 mM MgCl2.
DNA digestion was monitored by agarose gel.
ab270049 has not yet been referenced specifically in any publications.