Key features and details
- Mouse monoclonal [2D7] to Basophils
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Basophils antibody [2D7]
DescriptionMouse monoclonal [2D7] to Basophils
Species reactivityReacts with: Human
Lysate of purified Basophils.
- Chronic myeloid leukaemia (basophilia). Negative control: CD203c negative cells (Basophil depleted cell fractions).
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
RelevanceBasophils are multifunctional haematopoietic cells that secrete a wide variety of proinflammatory agents upon activation such as histamine and cytokines. Moreover, basophils can induce IgE production by B cells. Under normal conditions basophils are primarily produced in the bone marrow, whereas mature basophils reside in the circulation and can enter tissues at sites of inflammation. Both basophils and mast cells have been implicated in the pathogenesis of allergic inflammation due to the abundant expression of high affinity receptors for IgE. The 2D7 antibody can be used as a sensitive and precise marker for human basophils and does not react with lymphocytes, monocytes, eosinophils, neutrophils or mast cells in immunohistochemistry. Moreover, the antibody is suitable for western blot analysis of the 2D7 antigen.
ab155577 has been referenced in 6 publications.
- Hashimoto T et al. Pathophysiological mechanisms of itch in bullous pemphigoid. J Am Acad Dermatol N/A:N/A (2019). PubMed: 31351883
- Yanagawa M et al. Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis. J Gastroenterol 53:449-460 (2018). IHC-P ; Human . PubMed: 28921377
- Kim DK et al. MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis. PLoS One 9:e111352 (2014). IHC ; Human . PubMed: 25361058
- Agis H et al. Enumeration and immunohistochemical characterisation of bone marrow basophils in myeloproliferative disorders using the basophil specific monoclonal antibody 2D7. J Clin Pathol 59:396-402 (2006). PubMed: 16461568
- Plager DA et al. Identification of basophils by a mAb directed against pro-major basic protein 1. J Allergy Clin Immunol 117:626-34 (2006). PubMed: 16522463
- Irani AM et al. Immunohistochemical detection of human basophils in late-phase skin reactions. J Allergy Clin Immunol 101:354-62 (1998). PubMed: 9525452