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  1. Link

    basp1-antibody-ab214322.pdf

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Neuroscience Cell Type Marker Neuron marker Growth Cone
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Anti-BASP1 antibody (ab214322)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BASP1 antibody (ab214322)
  • Western blot - Anti-BASP1 antibody (ab214322)

Key features and details

  • Rabbit polyclonal to BASP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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View more associated products

Overview

  • Product name

    Anti-BASP1 antibody
    See all BASP1 primary antibodies
  • Description

    Rabbit polyclonal to BASP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human BASP1 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P80723

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain tissue; Mouse brain lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Neuroscience
    • Development

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • Mouse brain tissue lysate - total protein (ab4022)
  • Recombinant Protein

    • Recombinant Human BASP1 protein (ab140056)
  • Related Products

    • Recombinant Human BASP1 protein (ab140056)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab214322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/100 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P (1)
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/100 - 1/1000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    BASP1 is a brain abundant membrane bound protein with several transient phosphorylation sites and PEST motifs. BASP1 binds calmodulin, is phosphorylated by protein kinase C and undergoes N-terminal myristoylation. BASP1 is expressed specifically in nervous tissue. Intercellular signaling and nerve outgrowth are influenced by a number of proteins expressed at nerve endings and synapses of which BASP1 is one.
  • Cellular localization

    Cell membrane; Lipid-anchor. Cell projection; growth cone. Note: Associated with the membranes of growth cones that form the tips of elongating axons.
  • Database links

    • Entrez Gene: 10409 Human
    • Entrez Gene: 70350 Mouse
    • Entrez Gene: 64160 Rat
    • Omim: 605940 Human
    • SwissProt: P80723 Human
    • SwissProt: Q91XV3 Mouse
    • SwissProt: Q05175 Rat
    • Alternative names

      • 22 kDa neuronal tissue enriched acidic protein antibody
      • BASP 1 antibody
      • BASP1 protein antibody
      • Brain abundant membrane attached signal protein 1 antibody
      • Brain Abundant Signal Protein Membrane Attached 1 antibody
      • Brain Acid Soluble Protein 1 antibody
      • CAP 23 antibody
      • CAP23 antibody
      • MGC8555 antibody
      • NAP 22 antibody
      • NAP22 antibody
      • Neuronal axonal membrane protein NAP 22 antibody
      • Neuronal tissue enriched acidic protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BASP1 antibody (ab214322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BASP1 antibody (ab214322)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling with BASP1 with ab214322 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

    • Western blot - Anti-BASP1 antibody (ab214322)
      Western blot - Anti-BASP1 antibody (ab214322)
      Anti-BASP1 antibody (ab214322) at 1/200 dilution + Mouse brain lysate

      Secondary
      Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

      Predicted band size: 23 kDa



      15% gel

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab214322? Please let us know so that we can cite the reference in this datasheet.

    ab214322 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BASP1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain -glial cells, neurons.)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako, Target Retrieval Solution, pH 9
    Permeabilization
    No
    Specification
    brain -glial cells, neurons.
    Blocking step
    hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 0.3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Angela Cho

    Verified customer

    Submitted Jul 31 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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