Anti-Bassoon/BSN antibody (ab110426)
Key features and details
- Rabbit polyclonal to Bassoon/BSN
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Bassoon/BSN antibody
See all Bassoon/BSN primary antibodies -
Description
Rabbit polyclonal to Bassoon/BSN -
Host species
Rabbit -
Specificity
No cross-reactivity to Piccolo. Multiple isoforms can be detected. -
Tested applications
Suitable for: IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein (His-tag) corresponding to Human Bassoon/BSN aa 750-1050 (N terminal). N-terminal his-tagged fusion protein
Database link: Q9UPA5 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 416 kDa. For large isoforms reduce dilution.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 416 kDa. For large isoforms reduce dilution. |
Target
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Function
Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. -
Tissue specificity
Exclusively expressed in brain. -
Post-translational
modificationsMyristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization. -
Cellular localization
Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density. - Information by UniProt
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Database links
- Entrez Gene: 8927 Human
- Entrez Gene: 12217 Mouse
- Entrez Gene: 29138 Rat
- Omim: 604020 Human
- SwissProt: Q9UPA5 Human
- SwissProt: O88737 Mouse
- SwissProt: O88778 Rat
- Unigene: 194684 Human
see all -
Alternative names
- Bassoon protein antibody
- bsn antibody
- BSN_HUMAN antibody
see all
Images
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All lanes : Anti-Bassoon/BSN antibody (ab110426) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 416 kDa -
Immunofluorescence of Neuromuscular junction whole muscle samples from adult mouse, stained with ab110426 at 1/400 dilution. Secondary (Alexa 488 goat antimouse IgG, IgM (H+L). Note: bright staining of the NMJ very selectively and in some terminals, very brightly.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110426 has been referenced in 1 publication.
- Guo N et al. Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization. Nat Med 24:438-449 (2018). PubMed: 29529016