Overview

  • Product name

  • Description

    Rabbit polyclonal to Bassoon/BSN
  • Host species

    Rabbit
  • Specificity

    No cross-reactivity to Piccolo. Multiple isoforms can be detected.
  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Human Bassoon/BSN aa 786-1041.

  • Positive control

    • Mouse and Rat brain lysates; Neuromuscular junction whole muscle tissue from adult mouse.
  • General notes

     This product was previously labelled as Bassoon

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 416 kDa. For large isoforms reduce dilution.

Target

  • Function

    Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.
  • Tissue specificity

    Exclusively expressed in brain.
  • Post-translational
    modifications

    Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.
  • Cellular localization

    Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bassoon protein antibody
    • bsn antibody
    • BSN_HUMAN antibody
    • KIAA0434 antibody
    • Neuronal double zinc finger protein antibody
    • Presynaptic cytomatrix protein antibody
    • Protein bassoon antibody
    • Zinc finger protein 231 antibody
    • ZNF 231 antibody
    • ZNF231 antibody
    see all

Images

  • All lanes : Anti-Bassoon/BSN antibody (ab110426) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Mouse brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 416 kDa

  • Immunofluorescence of Neuromuscular junction whole muscle samples from adult mouse, stained with ab110426 at 1/400 dilution. Secondary (Alexa 488 goat antimouse IgG, IgM (H+L). Note: bright staining of the NMJ very selectively and in some terminals, very brightly.

References

This product has been referenced in:

  • Guo N  et al. Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization. Nat Med 24:438-449 (2018). Read more (PubMed: 29529016) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

We have 4 antibodies in our catalog raised against Bassoon: ab82958 (mouse), ab76065 (rabbit), ab110426 (rabbit) and ab101772 (goat).

Could you please let me know what kind of IHC you are planning to perform: IHC-P or IHC-Fr?
What is the species of your samples?
The mouse monoclonal has been tested in ICC, IP and WB. And the goat one has been tested in IHC-Fr and IHC-P.

Please let me know the requested details so that I can assist you further.

I look forward to hear back from you.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up