• Product name

  • Description

    Mouse polyclonal to BAT3/BAG-6
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human BAT3/BAG-6.
    Database link: NP_542433.1

  • Positive control

    • A-431 cell lysate with BAT3/BAG-6 expression, BAT3/BAG-6 transfected 293T cell lysate and HeLa cells.
  • General notes

    Previously labelled as BAT3. 



Our Abpromise guarantee covers the use of ab88292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Also involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
  • Sequence similarities

    Contains 1 ubiquitin-like domain.
  • Post-translational

    Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
    In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2410045D21Rik antibody
    • AA408914 antibody
    • BAG 6 antibody
    • BAG family molecular chaperone regulator 6 antibody
    • BAG-6 antibody
    • BAG6 antibody
    • BAG6_HUMAN antibody
    • BAT 3 antibody
    • BAT3 antibody
    • BCL2-associated athanogene 6 antibody
    • D17H6S52E antibody
    • D6S52E antibody
    • G3 antibody
    • HLA B associated transcript 3 antibody
    • HLA-B associated transcript 3 antibody
    • HLA-B associated transcript-3 antibody
    • HLA-B-associated transcript 3 antibody
    • large proline rich protein BAG6 antibody
    • Large proline rich protein BAT3 antibody
    • Large proline-rich protein BAG6 antibody
    • large proline-rich protein BAT3 antibody
    • Protein G3 antibody
    • Protein Scythe antibody
    • Scythe antibody
    • Scythe, homolog of Xenopus antibody
    see all


  • Anti-BAT3/BAG-6 antibody (ab88292) at 1 µg/ml + A-431 cell lysate with BAT3 / BAG-6 expression at 50 µg

    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 119 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-BAT3/BAG-6 antibody (ab88292) at 1 µg/ml

    Lane 1 : BAT3/BAG-6 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    All lanes : Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size: 119 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Immunofluorescence of ab88292 on HeLa cell with antibody concentration at 10 ug/ml
  • IHC image of ab88292 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88292, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As


Thank you for reporting the problem with ab88292 in IHC-P. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, I would like to offer some suggestions to help optimize the results. Ithink your protocol is absolutely fine. I would just suggest to changethe antigen retrieval method byusing Tris / EDTA pH 9.0 buffer. This buffer is by far the most efficient for most of thecases.

Should this suggestion not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up