Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-BAT3/BAG-6 antibody [EPR9223] (Phycoerythrin) (ab210838)

Overview

  • Product name

    Anti-BAT3/BAG-6 antibody [EPR9223] (Phycoerythrin)
    See all BAT3/BAG-6 primary antibodies
  • Description

    Rabbit monoclonal [EPR9223] to BAT3/BAG-6 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    corresponding to Human BAT3/BAG-6 aa 1100 to the C-terminus.
    Database link: P46379

  • Positive control

    • Flow Cytometry: HeLa cells, HAP1-WT cells.
  • General notes

    Previously labelled as BAT3. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab210838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Also involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
  • Sequence similarities

    Contains 1 ubiquitin-like domain.
  • Post-translational
    modifications

    Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
    In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex.
  • Cellular localization

    Cytoplasm > cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2410045D21Rik antibody
    • AA408914 antibody
    • BAG 6 antibody
    • BAG family molecular chaperone regulator 6 antibody
    • BAG-6 antibody
    • BAG6 antibody
    • BAG6_HUMAN antibody
    • BAT 3 antibody
    • BAT3 antibody
    • BCL2-associated athanogene 6 antibody
    • D17H6S52E antibody
    • D6S52E antibody
    • G3 antibody
    • HLA B associated transcript 3 antibody
    • HLA-B associated transcript 3 antibody
    • HLA-B associated transcript-3 antibody
    • HLA-B-associated transcript 3 antibody
    • large proline rich protein BAG6 antibody
    • Large proline rich protein BAT3 antibody
    • Large proline-rich protein BAG6 antibody
    • large proline-rich protein BAT3 antibody
    • Protein G3 antibody
    • Protein Scythe antibody
    • Scythe antibody
    • Scythe, homolog of Xenopus antibody
    see all

Images

  • Overlay histogram showing HAP1 wildtype (green line) and HAP1-BAG6 knockout cells (red line) stained with ab210838. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210838, 0.1µg/ml dilution) for 30 min at 22°C.

    A rabbit monoclonal IgG isotype control antibody  (ab209478) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-BAG6  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm)  and 586/15 bandpass filter.

    This antibody can also be used in HAP1 cells fixed with  4% formaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Overlay histogram showing HeLa cells stained with ab210838 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210838, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

References

ab210838 has not yet been referenced specifically in any publications.

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