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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-BATF antibody (ab196762)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF antibody (ab196762)

    Key features and details

    • Rabbit polyclonal to BATF
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human BATF protein (ab104928)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-BATF antibody
      See all BATF primary antibodies
    • Description

      Rabbit polyclonal to BATF
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide corresponding to Human BATF (internal sequence).
      Database link: Q16520

    • Positive control

      • Paraffin-embedded Human breast carcinoma tissue
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS

      PBS is without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • Leucine Zipper
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Polymerase associated factors
      • Pol II Transcription
      • Other
      • Epigenetics and Nuclear Signaling
      • Transcription
      • RNA polymerase

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human BATF protein (ab104928)
    • Related Products

      • Recombinant Human BATF protein (ab104928)

    Applications

    Our Abpromise guarantee covers the use of ab196762 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/50 - 1/100.

    Target

    • Function

      AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.
    • Tissue specificity

      Expressed at highest levels in lung, and at lower levels in placenta, liver, kidney, spleen, and peripheral blood. Detected in SW480 colorectal cancer cell line and several hematopoietic tumor cell lines, including Raji Burkitt's lymphoma. Strongly expressed in mature B- and T-lymphocytes. Also expressed in moderate levels in lymph node and appendix and at low levels in thymus and bone marrow (PubMed:10777209).
    • Sequence similarities

      Belongs to the bZIP family.
      Contains 1 bZIP (basic-leucine zipper) domain.
    • Post-translational
      modifications

      Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.
      Phosphorylated.
    • Cellular localization

      Nucleus. Cytoplasm. Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.
    • Target information above from: UniProt accession Q16520 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10538 Human
      • Entrez Gene: 53314 Mouse
      • Omim: 612476 Human
      • SwissProt: Q16520 Human
      • SwissProt: O35284 Mouse
      • Unigene: 509964 Human
      • Unigene: 6672 Mouse
      • Alternative names

        • Activating transcription factor B antibody
        • B ATF antibody
        • B-ATF antibody
        • B-cell-activating transcription factor antibody
        • Basic leucine zipper transcription factor like antibody
        • Basic leucine zipper transcriptional factor ATF like antibody
        • Basic leucine zipper transcriptional factor ATF-like antibody
        • Batf antibody
        • BATF_HUMAN antibody
        • BATF1 antibody
        • SF HT activated gene 2 protein antibody
        • SF-HT-activated gene 2 protein antibody
        • SFA 2 antibody
        • SFA-2 antibody
        • SFA2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF antibody (ab196762)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF antibody (ab196762)

        Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling BATF with ab196762 at 1/50 dilution. Right panel was preincubated with blocking peptide.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab196762? Please let us know so that we can cite the reference in this datasheet.

      ab196762 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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