Overview

  • Product name

    Anti-BATF antibody [EPR21911] - BSA and Azide free
    See all BATF primary antibodies
  • Description

    Rabbit monoclonal [EPR21911] to BATF - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human BATF aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q16520

  • Positive control

    • ICC/IF: KARPAS-299 cells.
  • General notes

    Ab234621 is the carrier-free version of ab221146. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234621 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters: regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs): up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs.
  • Tissue specificity

    Expressed at highest levels in lung, and at lower levels in placenta, liver, kidney, spleen, and peripheral blood. Detected in SW480 colorectal cancer cell line and several hematopoietic tumor cell lines, including Raji Burkitt's lymphoma. Strongly expressed in mature B- and T-lymphocytes. Also expressed in moderate levels in lymph node and appendix and at low levels in thymus and bone marrow (PubMed:10777209).
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues and at least one tyrosine residue. Phosphorylation at Ser-43 inhibit DNA binding activity and transforms it as a negative regulator of AP-1 mediated transcription.
    Phosphorylated.
  • Cellular localization

    Nucleus. Cytoplasm. Present in the nucleus and cytoplasm, but shows increased nuclear translocation after activation of T-cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating transcription factor B antibody
    • B ATF antibody
    • B-ATF antibody
    • B-cell-activating transcription factor antibody
    • Basic leucine zipper transcription factor like antibody
    • Basic leucine zipper transcriptional factor ATF like antibody
    • Basic leucine zipper transcriptional factor ATF-like antibody
    • Batf antibody
    • BATF_HUMAN antibody
    • BATF1 antibody
    • SF HT activated gene 2 protein antibody
    • SF-HT-activated gene 2 protein antibody
    • SFA 2 antibody
    • SFA-2 antibody
    • SFA2 antibody
    see all

Images

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A20 (mouse reticulum sarcoma) cell line labeling BATF with ab221146 at 1/500 (red) compared with a Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2,000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221146).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized KARPAS-299 (human anaplastic large cell lymphoma, right panel) or HeLa (human epithelial cell line from cervix adenocarcinoma, left panel) cell line labeling BATF with ab221146 at 1/500 (red) compared with a Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2,000 dilution was used as the secondary antibody.

    Negative control: HeLa cell line (PMID: 8570175).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221146).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF with ab221146 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing nuclear staining in KARPAS-299 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    PBS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution.

    Negative control: HeLa (human epithelial cell line from cervix adenocarcinoma) (PMID: 8570175).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221146).

References

ab234621 has not yet been referenced specifically in any publications.

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