Key features and details
- Rabbit polyclonal to BATF3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BATF3 antibody
See all BATF3 primary antibodies
DescriptionRabbit polyclonal to BATF3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
- WB: Jurkat whole cell and nuclear extracts. IP: Jurkat whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 14 kDa.|
|IP||1/100 - 1/500.|
RelevanceBATF3 belongs to the bZIP family and contains 1 bZIP domain. Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12-O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities.
- 21 kD small nuclear factor isolated from T cells antibody
- 21 kDa small nuclear factor isolated from T-cells antibody
- ATF like 3 antibody
All lanes : Anti-BATF3 antibody (ab229727) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
15% SDS-PAGE gel.
BATF3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 μg ab229727. Western blot was performed from the immunoprecipitate using ab229727. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab229727 IP in Jurkat whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229727 has not yet been referenced specifically in any publications.