Anti-BATF3 antibody (ab229727)
Key features and details
- Rabbit polyclonal to BATF3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BATF3 antibody
See all BATF3 primary antibodies -
Description
Rabbit polyclonal to BATF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human BATF3 aa 1 to the C-terminus.
Database link: Q9NR55 -
Positive control
- WB: Jurkat whole cell and nuclear extracts. IP: Jurkat whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
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IP |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
IP
1/100 - 1/500. |
Target
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Relevance
BATF3 belongs to the bZIP family and contains 1 bZIP domain. Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12-O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 55509 Human
- Omim: 612470 Human
- SwissProt: Q9NR55 Human
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Alternative names
- 21 kD small nuclear factor isolated from T cells antibody
- 21 kDa small nuclear factor isolated from T-cells antibody
- ATF like 3 antibody
see all
Images
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All lanes : Anti-BATF3 antibody (ab229727) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa15% SDS-PAGE gel.
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BATF3 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 μg ab229727. Western blot was performed from the immunoprecipitate using ab229727. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab229727 IP in Jurkat whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab229727 has been referenced in 1 publication.
- Li P et al. BATF3 promotes malignant phenotype of colorectal cancer through the S1PR1/p-STAT3/miR-155-3p/WDR82 axis. Cancer Gene Ther 28:400-412 (2021). PubMed: 33057139