Recombinant Anti-Bax antibody [5B7] (ab3191)
![Western blot - Anti-Bax antibody [5B7] (ab3191)](https://www.abcam.com/ps/products/3/ab3191/Images/ab3191-366359-AntiBax-antibody-5B7-western-blot-a20-c2c12-mouse.jpg "Western blot - Anti-Bax antibody [5B7] (ab3191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [5B7] to Bax
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Bax antibody [5B7]
See all Bax primary antibodies -
Description
Mouse monoclonal [5B7] to Bax -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A20 and C2C12 lysate. Flow Cyt (intra): RAW 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.5% BSA, PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5B7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1.043 µg/ml.
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| WB |
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
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| IP |
Use a concentration of 13.9 µg/ml.
|
| Notes |
|---|
|
Flow Cyt (Intra)
Use a concentration of 1.043 µg/ml. |
|
WB
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa. |
|
IP
Use a concentration of 13.9 µg/ml. |
Target
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Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 12028 Mouse
- SwissProt: Q07813 Mouse
- Unigene: 19904 Mouse
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Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
see all
Images
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Western blot - Anti-Bax antibody [5B7] (ab3191)Lane 1 : Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/ml
Lane 2 : Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/ml
Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
Lane 2 : C2C12 (Mouse myoblasts) lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 21 kDa
Exposure time: 26 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)](https://www.abcam.com/ps/products/3/ab3191/Images/ab3191-366360-AntiBax-antibody-5B7-flow-cytometry-raw2647-mouse.jpg)
Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)Intracellular flow cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043µg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.
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![Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)](https://www.abcam.com/ps/products/3/ab3191/Images/ab3191-366362-AntiBax-antibody-5B7-immunoprecipitation-raw2647-mouse.jpg)
Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 30 seconds. -
![Anti-Bax antibody [5B7] (ab3191)](https://www.abcam.com/ps/products/3/ab3191/Images/ab3191-4-benefits-of-recombinant-antibodies.png)
Anti-Bax antibody [5B7] (ab3191)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (10)
ab3191 has been referenced in 10 publications.
- Yu L et al. Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study. BMC Cancer 21:195 (2021). PubMed: 33632157
- Xiao J et al. Circular RNA CSNK1G1 promotes the progression of osteoarthritis by targeting the miR-4428/FUT2 axis. Int J Mol Med 47:232-242 (2021). PubMed: 33416120
- Wang M et al. Integrated study of miR-215 promoting breast cancer cell apoptosis by targeting RAD54B. J Cell Mol Med 25:3327-3338 (2021). PubMed: 33635591
- Duan N et al. Overexpression of HIPK2 removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells. J Cancer 12:1826-1837 (2021). PubMed: 33613771
- McKelvey KJ et al. Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma. Front Oncol 11:633210 (2021). PubMed: 33854970
- Thongchot S et al. Cancer-Associated Fibroblast-Derived IL-6 Determines Unfavorable Prognosis in Cholangiocarcinoma by Affecting Autophagy-Associated Chemoresponse. Cancers (Basel) 13:N/A (2021). PubMed: 33925189
- Liu Q et al. Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter. J Cancer 12:1990-1999 (2021). PubMed: 33753997
- Lu S & Lin CW Lentivirus-mediated transfer of gene encoding fibroblast growth factor-18 inhibits intervertebral disc degeneration. Exp Ther Med 22:856 (2021). PubMed: 34178129
- Cui X et al. miR-132 is upregulated in polycystic ovarian syndrome and inhibits granulosa cells viability by targeting Foxa1. Mol Med Rep 22:5155-5162 (2020). PubMed: 33174054
- Shi X et al. Persimmon oligomeric proanthocyanidins alleviate ultraviolet B-induced skin damage by regulating oxidative stress and inflammatory responses. Free Radic Res 54:765-776 (2020). PubMed: 33108915
