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Recombinant

Recombinant Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

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Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
  • Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
  • Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
  • Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5B7] to Bax - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-Bax antibody [5B7] - BSA and Azide free
    See all Bax primary antibodies

  • Description

    Mouse monoclonal [5B7] to Bax - BSA and Azide free

  • Host species

    Mouse

  • Specificity

    Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A20 and C2C12 lysate. Flow Cyt (intra): RAW 264.7 cells.

  • General notes

    ab252258 is the carrier-free version of ab3191.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab252258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use a concentration of 1.043 µg/ml.
WB
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
IP
Use a concentration of 13.9 µg/ml.
Notes
Flow Cyt (Intra)
Use a concentration of 1.043 µg/ml.
WB
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
IP
Use a concentration of 13.9 µg/ml.

Target

  • Function

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

  • Tissue specificity

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

  • Sequence similarities

    Belongs to the Bcl-2 family.

  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • Cellular localization

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Images

    • Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
      Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
      All lanes : Anti-Bax antibody [5B7] (ab3191) at 1/1000 dilution

      Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
      Lane 2 : C2C12 (Mouse myoblasts) lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa


      Exposure time: 26 seconds


      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
      Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191). 

      Intracellular Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at 1.043µg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution. 

       

       

    • Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
      Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

      Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml . Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
      Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
      Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
      Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)
      Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab252258? Please let us know so that we can cite the reference in this datasheet.

    ab252258 has not yet been referenced specifically in any publications.

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