Overview

  • Product name
    Anti-Bax antibody
    See all Bax primary antibodies
  • Description
    Rabbit polyclonal to Bax
  • Host species
    Rabbit
  • Specificity
    Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat, Dog, Pig, Macaque monkey
  • Immunogen

    Synthetic peptide within Human Bax aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q07812
    (Peptide available as ab117401)

  • Positive control
    • This antibody gave a positive signal in Human lymph node tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab104156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • Function
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificity
    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization
    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Images

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 4 and 6: Bax knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Green signal from target – ab104156 observed at 20 kDa
      Lanes 3 and 4: Red signal from loading control – ab8226 observed at 42 kDa
      Lanes 5 and 6: Merged (red and green) signal

      ab104156 was shown to react with Bax when Bax knockout samples were used, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab104156 and ab8226 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)
      Anti-Bax antibody (ab104156) at 1 µg/ml + Human lymph node tissue lysate - total protein (ab29871) at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 21 kDa


      Exposure time: 4 minutes
    • Western blot - Anti-Bax antibody (ab104156)
      Western blot - Anti-Bax antibody (ab104156)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Bax knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab104156 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

      This western blot image is a comparison between ab104156 and a competitor's top cited rabbit polyclonal antibody.

    References

    This product has been referenced in:
    • Zhang Z  et al. Resveratrol attenuates mechanical compression-induced nucleus pulposus cell apoptosis through regulating the ERK1/2 signaling pathway in a disc organ culture. Biosci Rep 38:N/A (2018). Read more (PubMed: 29439139) »

    See 1 Publication for this product

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