Anti-Bax antibody (ab199677)

Rabbit polyclonal Bax antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to Bax
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Bax aa 51-79. The exact sequence is proprietary.
    Database link: Q07812

  • Positive control

    • 293 and HT1080 cell lysates; HUVEC cells; Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab199677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100.

Target

  • Function

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificity

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Images

  • All lanes : Anti-Bax antibody (ab199677) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HT1080 cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 21 kDa

  • Immunofluorescent analysis of HUVEC cells labeling Bax using ab199677 at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded human liver cancer tissue labeling Bax using ab199677 at 1/50 dilution (left) and negative control (right).

References

This product has been referenced in:

  • Lee J  et al. CRISPR/Cas9 Edited sRAGE-MSCs Protect Neuronal Death in Parkinson?s Disease Model. Int J Stem Cells 12:114-124 (2019). Read more (PubMed: 30836725) »
  • Wang M  et al. Protective effects of Myrica rubra flavonoids against hypoxia/reoxygenation-induced cardiomyocyte injury via the regulation of the PI3K/Akt/GSK3ß pathway. Int J Mol Med 43:2133-2143 (2019). Read more (PubMed: 30864694) »
See all 3 Publications for this product

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