• Product name

  • Description

    Rabbit polyclonal to Bax
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Bax.
    Database link: Q07812

  • Positive control

    • WB: HEK293 whole cell lysate (ab7902), HeLa cell extracts. ICC/IF: A549 cells. IHC-P: Human lung carcinoma tissue. Human gallbladder tissue. ELISA: Primary cultured rat cortical neurons.



Our Abpromise guarantee covers the use of ab53154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ELISA 1/5000. PubMed: 22629415
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificity

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all


  • All lanes : Anti-Bax antibody (ab53154) at 1/500 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.

  • ab53154 staining human normal gallbladder tissue. Staining is localized to cell membrane and cytoplasm.
    Left panel: ab53154 at 4 µg/ml. Right panel: Isotype control.
    Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with the EDTA pH 9.0. Slides were blocked in 3% H2O2, methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ELISA analysis of primary cultured rat cortical neurons, detecting Bax using ab53154.
    Cells were treated with (Tat) or without Tat (control) and either Estradiol, Genistein or Daidzein. Lysates were prepared after 24 hours. A 96-well plate was coated using carbonate coating buffer. 20 µg cell lysate was added to wells and incubated overnight at 4°C. Plates were blocked with 1% BSA for 2 hours at room temperature. Primary antibody (1/5000) was added to sample wells before incubating overnight at 4°C. A goat anti-rabbit ALP-conjugated IgG was used as secondary antibody and phosphatase substrate mixture was added. Absorbance was read at 650nm.


This product has been referenced in:

  • Li J & Zheng J Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in vitro. Mol Med Rep 19:821-830 (2019). Read more (PubMed: 30569095) »
  • Xing Y & Li L Gastrodin protects rat cardiomyocytes H9c2 from hypoxia-induced injury by up-regulation of microRNA-21. Int J Biochem Cell Biol 109:8-16 (2019). Read more (PubMed: 30684569) »
See all 62 Publications for this product

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 05 2007

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