Recombinant Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)
![Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)](https://www.abcam.com/ps/products/289/ab289364/Images/ab289364-481446-antibaxantibodyhl236westernblothek293ta431helahepg2human.jpg "Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL236] to Bax - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Bax antibody [HL236] - BSA and Azide free
See all Bax primary antibodies -
Description
Rabbit monoclonal [HL236] to Bax - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Bax (N terminal). Conjugated to a carrier protein.
Database link: Q07812 -
Positive control
- IHC-P: Mouse kidney tissue. WB: PC-12, HEK-293T, HeLa, A431, HepG2 and Rat2 whole cell extracts.
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General notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL236 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/500 - 1/3000. Predicted molecular weight: 21 kDa.
|
| Notes |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 21 kDa. |
Target
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Function
Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
Cellular localization
Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 581 Human
- Entrez Gene: 12028 Mouse
- Entrez Gene: 24887 Rat
- Omim: 600040 Human
- SwissProt: Q07812 Human
- SwissProt: Q07813 Mouse
- SwissProt: Q63690 Rat
- Unigene: 624291 Human
see all -
Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
see all
Images
-
Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)All lanes : Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell extracts
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 21 kDaWestern blot analysis of various whole cell extracts, separated by 15% SDS-PAGE.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)](https://www.abcam.com/ps/products/289/ab289364/Images/ab289364-480885-AntiBax-antibody-HL236-BSA-and-Azide-free-mouse-kidney-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue staining for Bax using ab289364 diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
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![Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)](https://www.abcam.com/ps/products/289/ab289364/Images/ab289364-480889-Anti-Bax-antibody-HL236-BSA-and-Azide-free-whole-cell-extracts-western-blot-a.jpg)
Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)All lanes : Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution
Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell extracts
Lane 2 : Rat2 (Rat fibroblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 21 kDaWhole cell extracts separated by 15% SDS-PAGE
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![Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)](https://www.abcam.com/ps/products/289/ab289364/Images/ab289364-480998-benefitsofrecombinantantibodies.jpg)
Anti-Bax antibody [HL236] - BSA and Azide free (ab289364)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab289364 has not yet been referenced specifically in any publications.
