Recombinant Anti-Bax antibody [SP47] (ab81083)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP47] to Bax
Suitable for: IHC-P
Reacts with: Mouse, Human

Carrier Free

Product name

Anti-Bax antibody [SP47]
See all Bax primary antibodies
Description

Rabbit monoclonal [SP47] to Bax
Host species

Rabbit
Tested applications

Suitable for: IHC-Pmore details
Species reactivity

Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
Immunogen

Synthetic peptide within Human Bax aa 50-150 (internal sequence). The exact sequence is proprietary.
Database link: Q07812
Positive control
- IHC-P: Human breast, cervix, esophagus, prostate, skin, tonsil, skin squamous cell carcinoma, lung squamous cell carcinoma and HK lymphoma tissues.
General notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP47
Isotype

IgG
Research areas

Alternative Versions
- Anti-Bax antibody [SP47] - BSA and Azide free (ab240930)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Bax protein (ab173026)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab81083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature.

Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary antibody incubation for 30 minutes at room temperature.

Function

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
Tissue specificity

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Sequence similarities

Belongs to the Bcl-2 family.
Domain

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization

Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
Target information above from: UniProt accession Q07812 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 280730 Cow
- Entrez Gene: 581 Human
- Entrez Gene: 12028 Mouse
- Entrez Gene: 24887 Rat
- Omim: 600040 Human
- SwissProt: O02703 Cow
- SwissProt: Q07812 Human
- SwissProt: Q07813 Mouse
- SwissProt: Q63690 Rat
- Unigene: 624291 Human
- Unigene: 19904 Mouse
- Unigene: 10668 Rat
see all
Alternative names
- Apoptosis regulator BAX antibody
- BAX antibody
- Bax-protein antibody
- BAX_HUMAN antibody
- BAXA antibody
- Baxdelta2G9 antibody
- Baxdelta2G9omega antibody
- Baxdelta2omega antibody
- Bcl-2-like protein 4 antibody
- BCL2 associated X protein antibody
- BCL2 associated X protein omega antibody
- BCL2 associated X protein transcript variant delta2 antibody
- Bcl2-L-4 antibody
- BCL2L4 antibody
- membrane isoform alpha antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on mouse skin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Bax with ab81083 at 1/100 dilution (2.42 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human skin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human cervix tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human skin tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (ab81083)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.
Anti-Bax antibody [SP47] (ab81083)

Flow cytometry protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab81083? Please let us know so that we can cite the reference in this datasheet.

ab81083 has been referenced in 3 publications.

Chi C et al. Long Noncoding RNA SNHG7 Activates Wnt/ß-Catenin Signaling Pathway in Cervical Cancer Cells by Epigenetically Silencing DKK1. Cancer Biother Radiopharm 35:329-337 (2020). PubMed: 32275170
Zhang H et al. LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway. Cancer Cell Int 19:350 (2019). PubMed: 31889902
Florencio-Silva R et al. Effects of estrogen status in osteocyte autophagy and its relation to osteocyte viability in alveolar process of ovariectomized rats. Biomed Pharmacother 98:406-415 (2018). IHC-P ; Rat . PubMed: 29276969

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (3)

Customer reviews and Q&As