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    bax-phospho-s184-antibody-ab111391.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-Bax (phospho S184) antibody (ab111391)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax (phospho S184) antibody (ab111391)

    Key features and details

    • Rabbit polyclonal to Bax (phospho S184)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Recombinant Human Bax protein (ab173026)
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    Overview

    • Product name

      Anti-Bax (phospho S184) antibody
      See all Bax primary antibodies
    • Description

      Rabbit polyclonal to Bax (phospho S184)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rat
    • Immunogen

      Synthetic peptide within Human Bax (C terminal) (phospho S184). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: Q07812

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human brain tissue.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

      (without Mg2+ and Ca2+)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab111391 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins
      • Cell Biology
      • Apoptosis
      • Intracellular
      • Bcl2 Family
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Bcl 2 family
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Apoptosis marker and proteins ELISA kits
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Bcl 2 family
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Bax protein (ab173026)
    • Related Products

      • 2-Methoxyantimycin A3, Bcl-xL and Bcl-2 inhibitor (ab141523)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab111391 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • Tissue specificity

      Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • Sequence similarities

      Belongs to the Bcl-2 family.
    • Domain

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • Cellular localization

      Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Target information above from: UniProt accession Q07812 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 581 Human
      • Entrez Gene: 24887 Rat
      • Omim: 600040 Human
      • SwissProt: Q07812 Human
      • SwissProt: Q63690 Rat
      • Unigene: 624291 Human
      • Unigene: 10668 Rat
      • Alternative names

        • Apoptosis regulator BAX antibody
        • BAX antibody
        • Bax-protein antibody
        • BAX_HUMAN antibody
        • BAXA antibody
        • Baxdelta2G9 antibody
        • Baxdelta2G9omega antibody
        • Baxdelta2omega antibody
        • Bcl-2-like protein 4 antibody
        • BCL2 associated X protein antibody
        • BCL2 associated X protein omega antibody
        • BCL2 associated X protein transcript variant delta2 antibody
        • Bcl2-L-4 antibody
        • BCL2L4 antibody
        • membrane isoform alpha antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax (phospho S184) antibody (ab111391)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax (phospho S184) antibody (ab111391)

        Immunohistochemical analysis of paraffin-embedded Human brain tissue, staining Bax (phospho S184) with ab111391 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab111391? Please let us know so that we can cite the reference in this datasheet.

      ab111391 has been referenced in 5 publications.

      • Fan L & Zhou L AG490 protects cerebral ischemia/reperfusion injury via inhibiting the JAK2/3 signaling pathway. Brain Behav 11:e01911 (2021). PubMed: 33098244
      • Kim M  et al. Lycopene induces apoptosis by inhibiting nuclear translocation of ß-catenin in gastric cancer cells. J Physiol Pharmacol 70:N/A (2019). PubMed: 31741457
      • Kale J  et al. Phosphorylation switches Bax from promoting to inhibiting apoptosis thereby increasing drug resistance. EMBO Rep 19:N/A (2018). PubMed: 29987135
      • Tu SH  et al. Protein phosphatase Mg2+/Mn2+ dependent 1F promotes smoking-induced breast cancer by inactivating phosphorylated-p53-induced signals. Oncotarget 7:77516-77531 (2016). PubMed: 27769050
      • Xin M  et al. Small-molecule Bax agonists for cancer therapy. Nat Commun 5:4935 (2014). Mouse . PubMed: 25230299

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