Overview

  • Product name

    Anti-BAZ2A/TIP5 antibody - ChIP Grade
    See all BAZ2A/TIP5 primary antibodies
  • Description

    Rabbit polyclonal to BAZ2A/TIP5 - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human BAZ2A/TIP5.
    Database link: QU9IF9

  • Positive control

    • HEK293T, NIH 3T3 and HeLa cell extacts.
  • General notes

     This product was previously labelled as BAZ2A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab195278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

5µl per ChIP

WB 1/1000. Predicted molecular weight: 211 kDa.

Target

  • Relevance

    BAZ2A is a bromodomain adjacent to a zinc finger domain protein. It may play a role in transcriptional regulation interacting with ISWI and may serve a specific role in maintaining or altering the chromatin structure of the rDNA locus. BAz2A colocalizes with the basal RNA polymerase I transcription factor UBF in the nucleolus.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Bromodomain adjacent to zinc finger domain 2A antibody
    • Tip5 antibody
    • Transcription termination factor I interacting protein 5 antibody
    • TTF I interacting protein 5 antibody
    • WALp3 antibody
    see all

Images

  • All lanes : Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278) at 1/1000 dilution

    Lane 1 : NIH 3T3 nuclear extract
    Lane 2 : HeLa nuclear extract

    Lysates/proteins at 150 µg per lane.

    Predicted band size: 211 kDa

  • ChIP assays were performed using ab195278. Chromatin from HEK293T and NIH 3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 µg sheared chromatin and either 5 µl ab195278 or 5 µl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure shows the recovery by ab195278 and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH 3T3 cells, BAZ2A/TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

References

This product has been referenced in:

  • Li C  et al. The transcription factor 7 like 2-binding protein TIP5 activates ß-catenin/transcription factor signaling in hepatocellular carcinoma. Mol Med Rep 17:7645-7651 (2018). Read more (PubMed: 29620186) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEF)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
14 µg
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 14 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Permeabilization
Yes - methanol
Specification
MEFs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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