Key features and details
- Rabbit polyclonal to BAZ2A/TIP5 - ChIP Grade
- Suitable for: ChIP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-BAZ2A/TIP5 antibody - ChIP Grade
See all BAZ2A/TIP5 primary antibodies
DescriptionRabbit polyclonal to BAZ2A/TIP5 - ChIP Grade
Tested applicationsSuitable for: ChIP, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human BAZ2A/TIP5.
Database link: QU9IF9
- HEK293T, NIH 3T3 and HeLa cell extacts.
This product was previously labelled as BAZ2A
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab195278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.
5µl per ChIP
|WB||1/1000. Predicted molecular weight: 211 kDa.|
RelevanceBAZ2A is a bromodomain adjacent to a zinc finger domain protein. It may play a role in transcriptional regulation interacting with ISWI and may serve a specific role in maintaining or altering the chromatin structure of the rDNA locus. BAz2A colocalizes with the basal RNA polymerase I transcription factor UBF in the nucleolus.
- Bromodomain adjacent to zinc finger domain 2A antibody
- Tip5 antibody
- Transcription termination factor I interacting protein 5 antibody
All lanes : Anti-BAZ2A/TIP5 antibody - ChIP Grade (ab195278) at 1/1000 dilution
Lane 1 : NIH 3T3 nuclear extract
Lane 2 : HeLa nuclear extract
Lysates/proteins at 150 µg per lane.
Predicted band size: 211 kDa
ChIP assays were performed using ab195278. Chromatin from HEK293T and NIH 3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 µg sheared chromatin and either 5 µl ab195278 or 5 µl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure shows the recovery by ab195278 and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH 3T3 cells, BAZ2A/TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.
ab195278 has been referenced in 1 publication.
- Li C et al. The transcription factor 7 like 2-binding protein TIP5 activates ß-catenin/transcription factor signaling in hepatocellular carcinoma. Mol Med Rep 17:7645-7651 (2018). PubMed: 29620186