Overview

  • Product name

    Anti-BBF2H7 antibody - N-terminal
    See all BBF2H7 primary antibodies
  • Description

    Goat polyclonal to BBF2H7 - N-terminal
  • Host species

    Goat
  • Specificity

    ab223640 is expected to recognize both reported isoforms (NP_919047.2; NP_001240704.1).
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human BBF2H7 aa 15-28 (N terminal) (Cysteine residue). (NP_919047.2).
    Sequence:

    DRKLSELSEPGDGE-C


    Database link: Q70SY1

  • Positive control

    • IHC-P: Human placenta tissue. WB: Human placenta lysate.
  • General notes

     This product was previously labelled as CREB3L2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab223640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 57, 28 kDa).

Target

  • Function

    Transcriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.
  • Tissue specificity

    Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.
  • Involvement in disease

    A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
  • Sequence similarities

    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP (basic-leucine zipper) domain.
  • Post-translational
    modifications

    Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
  • Cellular localization

    Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B ZIB transcription factor antibody
    • Basic transcription factor 2 antibody
    • BBF2 human homolog on chromosome 7 antibody
    • BBF2H7 antibody
    • BZIB transcription factor antibody
    • cAMP responsive element binding protein 3 like 2 antibody
    • cAMP-responsive element-binding protein 3-like protein 2 antibody
    • CR3L2_HUMAN antibody
    • Creb3l2 antibody
    • Cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • FUS/BBF2H7 protein antibody
    • MGC131709 antibody
    • MGC71006 antibody
    • Processed cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
    • TCAG_1951439 antibody
    see all

Images

  • Paraffin-embedded human placenta tissue stained for BBF2H7 using ab223640 at 5 µg/ml in immunohistochemical analysis, followed by AP staining.

  • Anti-BBF2H7 antibody - N-terminal (ab223640) at 1 µg/ml + Human placenta lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 57, 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab223640 has not yet been referenced specifically in any publications.

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